anti-Mouse (Murine) CHEK2 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • CHK-2
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • LOC396525
Epitope
AA 50-90, pThr68
51
51
39
36
19
17
15
15
14
13
12
10
8
8
7
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
487
218
198
19
19
16
13
8
5
4
2
1
1
1
1
1
1
Host
Rabbit
352
136
8
1
1
Clonality
Polyclonal
Conjugate
This CHEK2 antibody is un-conjugated
10
9
9
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
399
126
124
106
90
65
48
43
27
6
6
5
2
1
1
Options
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Catalog No. ABIN703165
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN685867 IF (p) IHC (p) WB Rabbit IgG AA 120-160 Log in to see Polyclonal 1
1 ABIN2811610 IF (p) Alexa Fluor 594 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN703172 IF (p) FITC Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703168 IF (p) Cy3 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703169 IF (p) Cy5 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703170 IF (p) Cy5.5 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703171 IF (p) Cy7 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN685874 IF (p) FITC Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN685870 IF (p) Cy3 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN685871 IF (p) Cy5 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN685873 IF (p) Cy7 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN685872 IF (p) Cy5.5 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN878462 IF (p) Alexa Fluor 350 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN890468 IF (p) Alexa Fluor 555 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN890470 IF (p) Alexa Fluor 488 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN890469 IF (p) Alexa Fluor 350 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN878463 IF (p) Alexa Fluor 488 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN878464 IF (p) Alexa Fluor 555 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN878465 IF (p) Alexa Fluor 647 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN890471 IF (p) Alexa Fluor 647 Rabbit IgG AA 120-160 Log in to see Polyclonal

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 50-90, pThr68
(51), (51), (39), (36), (19), (17), (15), (15), (14), (13), (12), (10), (8), (8), (7), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(487), (218), (198), (19), (19), (16), (13), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(352), (136), (8), (1), (1)
Clonality
Polyclonal
Conjugate This CHEK2 antibody is un-conjugated
(10), (9), (9), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(399), (126), (124), (106), (90), (65), (48), (43), (27), (6), (6), (5), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling ProductDetails: References for anti-CHEK2 antibody (ABIN703165) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of Thr68
Isotype IgG

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling ProductDetails: References for anti-CHEK2 antibody (ABIN703165) Images back to top
Antigen
Alternative Name Chk2 (CHEK2 Antibody Abstract)
Background

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.

Subcellular location: Nucleus

Synonyms: CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2, CHEK2

Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling ProductDetails: References for anti-CHEK2 antibody (ABIN703165) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details ProductDetails: References for anti-CHEK2 antibody (ABIN703165) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

ProductDetails: References for anti-CHEK2 antibody (ABIN703165)

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling Images back to top
Product cited in:

Yin, Jiang, Peng, Cui, Zhou, He, Zuo, Ouyang, Fan, Fang: "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." in: Oncotarget, Vol. 7, Issue 24, pp. 35592-35606, 2016

Guo, Cui, Peng, Fang, Zuo, Deng, Wang, Wu, Chen, Deng: "Dietary NiCl₂ causes G₂/M cell cycle arrest in the broiler's kidney." in: Oncotarget, Vol. 6, Issue 34, pp. 35964-77, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Chicken).

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling ProductDetails: References for anti-CHEK2 antibody (ABIN703165) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 50-90), (pThr68) antibody (ABIN703165) Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Phosp...
SDS-PAGE (SDS) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 50-90), (pThr68) antibody (ABIN703165) L1 rat brain lysates, L2 human colon carcinoma lysates probed with Anti- Phospho-CHK2...