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anti-Human E2F1 antibody for Flow Cytometry

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Epitope
AA 69-223
31
28
27
25
25
15
13
13
10
10
9
9
9
8
8
7
7
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
350
71
29
9
4
3
3
3
1
1
1
Host
Mouse
274
65
19
1
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
273
158
75
54
54
50
41
36
23
12
10
2
2
1
1
1
Supplier
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Catalog No. ABIN969516
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30464333 ABIN1845545 FACS IHC ELISA WB Mouse IgG1 AA 69-223 Log in to see
0.0010463598 ABIN1491636 FACS IHC ELISA WB Mouse IgG1 Log in to see 8G9
0.0010463598 ABIN2256853 FACS IF ELISA WB Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010463598 ABIN2256885 FACS IF ELISA WB APC Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010463598 ABIN2256887 FACS IF ELISA WB FITC Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010463598 ABIN2256889 FACS IF ELISA WB PE Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010463598 ABIN2256847 FACS IF ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010463598 ABIN2256886 FACS IF ELISA WB Biotin Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010463598 ABIN2256900 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B231
0.0010463598 ABIN4306767 ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G9

Top referenced anti-E2F1 antibody for Flow Cytometry

Similar anti-E2F1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 23 Antibodies 153 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 12 Antibodies
Flow Cytometry (FACS) 3 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 15 Antibodies 27 Antibodies
Immunofluorescence (IF) 18 Antibodies 46 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 3 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Western Blotting (WB) 70 Antibodies
Dot Blot (DB) 41 Antibodies
Gel Shift (GS) 2 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 69-223
(31), (28), (27), (25), (25), (15), (13), (13), (10), (10), (9), (9), (9), (8), (8), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(350), (71), (29), (9), (4), (3), (3), (3), (1), (1), (1)
Host Mouse
(274), (65), (19), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(273), (158), (75), (54), (54), (50), (41), (36), (23), (12), (10), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN969516) Images
Purification Purified
Immunogen Purified recombinant fragment of human E2F1 (AA 69-223) expressed in E. coli.
Clone 8G9
Isotype IgG1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN969516) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Molecular Weight 46.9 kDa
Gene ID 1869
HGNC 1869
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN969516) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN969516) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-E2F1 Antibody (ABIN969516)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Product cited in:

Okazaki, Matsunaga, Okazaki, Utoguchi, Suzuki, Maruyama, Koyanagi, Ohdo: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." in: Cancer research, Vol. 70, Issue 15, pp. 6238-46, 2010

Gurtner, Fuschi, Martelli, Manni, Artuso, Simonte, Ambrosino, Antonini, Folgiero, Falcioni, Sacchi, Piaggio: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." in: Cancer research, Vol. 70, Issue 23, pp. 9711-20, 2010

Locatelli, Bosotti, Ciomei, Brasca, Calogero, Mercurio, Fiorentini, Bertolotti, Scacheri, Scaburri, Galvani, Pesenti, De Baere, Soria, Lazar, Isacchi: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." in: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, 2010

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN969516) back to top
Supplier Images
ELISA image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 2: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and neg...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 5: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), a...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 6: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein...