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anti-Rat (Rattus) E2F1 antibody for Western Blotting

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Epitope
N-Term
31
28
27
25
25
15
14
13
10
10
9
9
9
8
8
7
7
6
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
358
74
28
9
4
3
2
2
1
Host
Rabbit
276
71
19
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
281
161
80
60
56
54
41
36
27
12
11
2
2
2
1
1
Supplier
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Catalog No. ABIN2792603
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN967439 IP WB Mouse IgG2a Log in to see KH95-E2F 5
0.13341247 ABIN2704740 WB Rabbit C-Term, pSer433 Log in to see Polyclonal
0.13341247 ABIN2704741 IHC WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN2460125 ELISA WB Rabbit Log in to see Polyclonal
0.13341247 ABIN3022840 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN670686 IF (p) IHC (p) WB Rabbit IgG AA 105-135 Log in to see Polyclonal
0.0011728023 ABIN2736667 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1585777 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011728023 ABIN1085506 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1085505 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN3222878 WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN1386153 IF (p) IHC (p) WB Rabbit IgG pSer375 Log in to see Polyclonal
0.0011728023 ABIN1386489 IF (p) IHC (p) WB Rabbit IgG pThr433 Log in to see Polyclonal
0.0011728023 ABIN2749846 ELISA WB FITC Rabbit IgG AA 45-85, N-Term Log in to see Polyclonal
0.0011728023 ABIN2749847 ELISA WB FITC Rabbit IgG AA 125-165, Middle Region Log in to see Polyclonal
0.0011728023 ABIN2749848 ELISA WB FITC Rabbit IgG AA 380-430, C-Term Log in to see Polyclonal
0.0011728023 ABIN2746554 ELISA WB Rabbit IgG AA 45-85, N-Term Log in to see Polyclonal
0.0011728023 ABIN2746555 ELISA WB Rabbit IgG AA 125-165, Middle Region Log in to see Polyclonal
0.0011728023 ABIN4306766 IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN2985992 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-E2F1 antibody for Western Blotting

Similar anti-E2F1 Antibodies

Application / Reactivity Human Mouse (Murine) Cow (Bovine) Dog (Canine) Chicken Rat (Rattus) Xenopus laevis
Western Blotting (WB) 282 Antibodies 72 Antibodies 3 Antibodies 3 Antibodies 1 Antibodies 26 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Immunoprecipitation (IP) 60 Antibodies 18 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies 23 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 80 Antibodies 33 Antibodies 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies 1 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope N-Term
(31), (28), (27), (25), (25), (15), (14), (13), (10), (10), (9), (9), (9), (8), (8), (7), (7), (6), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(358), (74), (28), (9), (4), (3), (2), (2), (1)
Host Rabbit
(276), (71), (19), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(281), (161), (80), (60), (56), (54), (41), (36), (27), (12), (11), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN2792603) Images
Sequence PARGRGRHPG KGVKSPGEKS RYETSLNLTT KRFLELLSHS ADGVVDLNWA
Cross-Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 80 %
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human E2F1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN2792603) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Molecular Weight 47 kDa
Gene ID 1869
NCBI Accession NM_005225, NP_005216
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN2792603) Images back to top
Application Notes WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 437 AA

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN2792603) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-E2F1 Antibody (ABIN2792603)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

Hu, Sun, Nadiminty, Lou, Pinder, Gao: "Transcriptional regulation of human RANK ligand gene expression by E2F1." in: Biochemical and biophysical research communications, Vol. 370, Issue 3, pp. 440-4, 2008

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN2792603) back to top
Supplier Images
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (N-Term) (ABIN2792603) WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...