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anti-Rat (Rattus) E2F1 antibody for Western Blotting

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
N-Term
30
30
30
27
25
15
14
14
12
11
10
9
9
9
8
8
6
6
6
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
365
97
42
9
8
5
4
4
4
3
3
3
2
1
1
Host
Rabbit
280
73
20
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
284
162
78
60
58
54
42
39
25
13
12
3
2
2
1
1
1
Options
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Catalog No. ABIN2792603
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN967439 IP WB Mouse IgG2a Log in to see KH95-E2F 5
3.0726016 ABIN2704740 WB Rabbit C-Term, pSer433 Log in to see Polyclonal
3.0726016 ABIN2704741 IHC WB Rabbit C-Term Log in to see Polyclonal
3.0726016 ABIN2460125 ELISA WB Rabbit Log in to see Polyclonal
3.0726016 ABIN2736667 IHC WB Rabbit IgG Log in to see Polyclonal
3.0726016 ABIN4903534 IF IP WB Mouse IgG Log in to see
3.0726016 ABIN3222878 WB Rabbit Log in to see Polyclonal
3.0726016 ABIN3022840 IHC WB Rabbit IgG Log in to see Polyclonal
3.0726016 ABIN4306766 IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.0726016 ABIN3030787 WB Rabbit IgG Middle Region Log in to see Polyclonal
1 ABIN216346 WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN464186 WB Rabbit IgG AA 107-156 Log in to see Polyclonal
1 ABIN670686 IF (p) IHC (p) WB Rabbit IgG AA 105-135 Log in to see Polyclonal
1 ABIN2620199 WB Rabbit IgG AA 246-260 Log in to see Polyclonal
1 ABIN1585777 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4901417 ELISA WB Rabbit IgG 2meLys80 Log in to see Polyclonal
1 ABIN4901415 ELISA WB Rabbit IgG acLys521 Log in to see Polyclonal
1 ABIN4901418 ELISA WB Mouse IgG2b Log in to see 4G8-2C2-C12
1 ABIN4901416 ELISA WB Rabbit IgG 3meLys5 Log in to see Polyclonal
1 ABIN2886195 WB Rabbit pThr433 Log in to see Polyclonal

Top referenced anti-E2F1 antibody for Western Blotting

Similar anti-E2F1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine) Cow (Bovine) Dog (Canine) Chicken Xenopus laevis
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies 1 Antibodies
Western Blotting (WB) 285 Antibodies 93 Antibodies 5 Antibodies 6 Antibodies 2 Antibodies 40 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 60 Antibodies 22 Antibodies 4 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope N-Term
(30), (30), (30), (27), (25), (15), (14), (14), (12), (11), (10), (9), (9), (9), (8), (8), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(365), (97), (42), (9), (8), (5), (4), (4), (4), (3), (3), (3), (2), (1), (1)
Host Rabbit
(280), (73), (20), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(284), (162), (78), (60), (58), (54), (42), (39), (25), (13), (12), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN2792603) Images
Sequence PARGRGRHPG KGVKSPGEKS RYETSLNLTT KRFLELLSHS ADGVVDLNWA
Cross-Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 80 %
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human E2F1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN2792603) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Molecular Weight 47 kDa
Gene ID 1869
NCBI Accession NM_005225, NP_005216
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN2792603) Images back to top
Application Notes WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 437 AA

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN2792603) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-E2F1 Antibody (ABIN2792603)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

Hu, Sun, Nadiminty, Lou, Pinder, Gao: "Transcriptional regulation of human RANK ligand gene expression by E2F1." in: Biochemical and biophysical research communications, Vol. 370, Issue 3, pp. 440-4, 2008

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN2792603) back to top
Supplier Images
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (N-Term) (ABIN2792603) WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...