anti-Xenopus laevis E2F1 antibody for Western Blotting

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
AA 1-89, AA 342-386
31
30
30
28
25
15
14
14
12
11
11
10
9
9
8
7
7
6
6
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Xenopus laevis
374
102
47
10
8
6
6
5
5
4
3
3
2
1
Host
Mouse
289
73
21
1
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
21
19
16
14
13
13
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
293
167
78
60
60
55
42
39
25
13
12
3
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN210874
$ 903.83
Plus shipping costs $45.00

Similar anti-E2F1 Antibodies

Application / Reactivity Xenopus laevis Human
Immunoprecipitation (IP) 1 Antibodies 61 Antibodies
Western Blotting (WB) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 39 Antibodies
ELISA 158 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 13 Antibodies
Gel Shift (GS) 3 Antibodies
Immunocytochemistry (ICC) 25 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 42 Antibodies
Immunohistochemistry (IHC) 78 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 60 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 1-89, AA 342-386
(31), (30), (30), (28), (25), (15), (14), (14), (12), (11), (11), (10), (9), (9), (8), (7), (7), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Xenopus laevis
(374), (102), (47), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1)
Host Mouse
(289), (73), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(21), (19), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(293), (167), (78), (60), (60), (55), (42), (39), (25), (13), (12), (3), (2), (2), (1), (1), (1)
Supplier Log in to see

Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling Images
Specificity Recognizes human E2F-1 at 55kD. Species cross-reactivity: Xenopus.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Rat, Elephant (94%) Opossum (89%) Chicken, Lizard (83%).
Purification Protein G purified
Immunogen GST fusion protein corresponding to full-length human E2F1 (Accession #NP_005216). Epitope: aa1-89 for clone KH20, aa342-386 for clone KH95.

Type of Immunogen: Fusion protein
Clone KH20-KH95
Isotype IgG

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background Name/Gene ID: E2F1

Synonyms: E2F1, E2F transcription factor 1, PRB-binding protein E2F-1, RBAP-1, RBBP3, RBBP-3, Transcription factor E2F1, RBAP1, E2F-1, PBR3
Gene ID 1869
NCBI Accession NP_005216
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling Images back to top
Application Notes Approved: IP, WB

Usage: Western Blot Analysis: 0.5-2 μg/mL of antibody detected E2F-1 (~55kD) in a HeLa nuclear extract. Positive Antigen Control: HeLa nuclear extract. Use 20 μg per lane. Immunoprecipitation: 4 μg of a previous lot of antibody immunoprecipitated E2F-1 from HeLa nuclear extract.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.