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anti-Human GADD45A antibody for ELISA

Recommended GADD45A Antibody (supplied by: Log in to see )

Antigen
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
  • AA545191
  • ddit1
  • Ddit1
  • DDIT1
  • Gadd45
  • GADD45
  • gadd45
  • gadd45a
  • GADD45A
  • MGC53491
  • zgc:91795
Epitope
AA 76-165
17
14
8
6
3
2
2
2
1
1
1
1
1
Reactivity
Human
80
30
29
Host
Mouse
67
14
Clonality
Monoclonal
Conjugate
This GADD45A antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
61
19
12
11
5
4
2
1
1
1
Supplier
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Catalog No. ABIN393701
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.001221279 ABIN560584 PLA ELISA WB Mouse IgG1 kappa AA 76-165, partial Log in to see 3D12 2
0.001221279 ABIN2271436 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.001221279 ABIN2271439 ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.001221279 ABIN2437252 FACS ELISA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.001221279 ABIN2439390 ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.001221279 ABIN1086821 ELISA WB Rabbit IgG Log in to see Polyclonal
0.001221279 ABIN1086822 ELISA WB Rabbit IgG Log in to see Polyclonal
0.001221279 ABIN1827731 ELISA WB Mouse IgG1, kappa Log in to see 3D12
0.001221279 ABIN2423505 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.001221279 ABIN2430149 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.001221279 ABIN4901578 ELISA WB Mouse IgG1 Log in to see 6C3-G3-E9
0.001221279 ABIN2271437 ELISA WB Mouse IgG1 kappa AA 76-165 Log in to see 3D12
0.001221279 ABIN2753641 ELISA WB HRP Rabbit IgG AA 1-165 Log in to see Polyclonal
0.001221279 ABIN2753642 ELISA WB FITC Rabbit IgG AA 1-165 Log in to see Polyclonal
0.001221279 ABIN2753643 ELISA WB Biotin Rabbit IgG AA 1-165 Log in to see Polyclonal
0.001221279 ABIN560583 ELISA Mouse AA 76-165, partial Log in to see Polyclonal
0.001221279 ABIN2417461 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.001221279 ABIN2753640 ELISA WB Rabbit IgG AA 1-165 Log in to see Polyclonal
0.001221279 ABIN2142249 ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-GADD45A antibody for ELISA

Similar anti-GADD45A Antibodies

Application / Reactivity Human
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 12 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 62 Antibodies

General

Antigen Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
Epitope AA 76-165
(17), (14), (8), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(80), (30), (29)
Host Mouse
(67), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This GADD45A antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(61), (19), (12), (11), (5), (4), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-GADD45A Antibody

Target Details GADD45A Application Details Handling References for anti-GADD45A Antibody (ABIN393701) Images
Specificity GADD45A (AAH11757, 76 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen GADD45A (AAH11757, 76 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 3D12
Isotype IgG1 kappa

Target Details GADD45A

Product Details anti-GADD45A Antibody Application Details Handling References for anti-GADD45A Antibody (ABIN393701) Images back to top
Antigen
Alternative Name GADD45A (GADD45A Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 18336 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-GADD45A Antibody Target Details GADD45A Handling References for anti-GADD45A Antibody (ABIN393701) Images back to top
Application Notes ELISA ~~ 1ug/ml~3ng/ml Western blot ~~ 1:500~1000
Comment

Background: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.

Restrictions For Research Use only

Handling

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details References for anti-GADD45A Antibody (ABIN393701) Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-GADD45A Antibody (ABIN393701)

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling Images back to top
Background publications

Sánchez, Pantoja-Uceda, Prieto, Diercks, Marcaida, Montoya, Campos-Olivas, Blanco: "Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase." in: The Journal of biological chemistry, Vol. 285, Issue 29, pp. 22196-201, 2010

van Roon, van Puijenbroek, Middeldorp, van Eijk, de Meijer, Erasmus, Wouters, van Engeland, Oosting, Hes, Tops, van Wezel, Boer, Morreau: "Early onset MSI-H colon cancer with MLH1 promoter methylation, is there a genetic predisposition?" in: BMC cancer, Vol. 10, pp. 180, 2010

Li, Zhao, Yin, Gao, Wu, Luo, Zhao, Zhang, Su, Hu, Long, Richardson, Lu: "Overexpression of the growth arrest and DNA damage-induced 45alpha gene contributes to autoimmunity by promoting DNA demethylation in lupus T cells." in: Arthritis and rheumatism, Vol. 62, Issue 5, pp. 1438-47, 2010

Yu, Di, Li, Rao, Fan, Yuan, Hu, Wu, Shen, Huang, Shao: "Genetic contribution of GADD45A to susceptibility to sporadic and non-BRCA1/2 familial breast cancers: a systematic evaluation in Chinese populations." in: Breast cancer research and treatment, Vol. 121, Issue 1, pp. 157-67, 2010

Young, Hopkins, Hay, Epton, Mills, Black, Gardner, Sullivan, Gamble: "A gene-based risk score for lung cancer susceptibility in smokers and ex-smokers." in: Postgraduate medical journal, Vol. 85, Issue 1008, pp. 515-24, 2009