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anti-Human GADD45A antibody for Flow Cytometry

Recommended GADD45A Antibody (supplied by: Log in to see )

Antigen
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
  • AA545191
  • ddit1
  • Ddit1
  • DDIT1
  • Gadd45
  • GADD45
  • gadd45
  • gadd45a
  • GADD45A
  • MGC53491
  • zgc:91795
Epitope
AA 133-165, C-Term
13
13
10
7
4
2
2
1
1
1
1
1
Reactivity
Human
76
29
28
Host
Rabbit
64
13
Clonality
Polyclonal
Conjugate
This GADD45A antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
55
19
11
11
5
4
2
1
1
1
Supplier
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Catalog No. ABIN650724
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.2896173 ABIN2437252 FACS ELISA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.0010723837 ABIN389281 FACS WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
0.0010723837 ABIN1812833 FACS WB Rabbit AA 136-165 Log in to see Polyclonal
0.0010723837 ABIN1840358 FACS WB Rabbit N-Term Log in to see Polyclonal
0.0010723837 ABIN2796450 FACS WB Rabbit Ig Fraction C-Term Log in to see Polyclonal

Top referenced anti-GADD45A antibody for Flow Cytometry

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Application / Reactivity Human
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 56 Antibodies

General

Antigen Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
Epitope AA 133-165, C-Term
(13), (13), (10), (7), (4), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(76), (29), (28)
Host Rabbit
(64), (13)
Clonality (Clone)
Polyclonal   ( )
Conjugate This GADD45A antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(55), (19), (11), (11), (5), (4), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-GADD45A Antibody

Target Details GADD45A Application Details Handling References for anti-GADD45A Antibody (ABIN650724) Images
Specificity This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136~165 amino acids from the C-terminal region of human GADD45A.
Predicted Reactivity Cow (Bovine),Hamster,Mouse (Murine),Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 AA from the C-terminal region of human GADD45A.
Clone RB16521
Isotype Ig

Target Details GADD45A

Product Details anti-GADD45A Antibody Application Details Handling References for anti-GADD45A Antibody (ABIN650724) Images back to top
Antigen
Alternative Name GADD45A (GADD45A Antibody Abstract)
Background GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.
Synonyms: Growth arrest and DNA-damage-inducible protein GADD45 alpha, DNA-damage-inducible transcript 1, DDIT-1, GADD45A, DDIT1, GADD45
Molecular Weight 18205 DA
Gene ID 1647
UniProt P24522
Research Area Stem Cells, Protein Modifications
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-GADD45A Antibody Target Details GADD45A Handling References for anti-GADD45A Antibody (ABIN650724) Images back to top
Application Notes WB = 1:1000, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details References for anti-GADD45A Antibody (ABIN650724) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-GADD45A Antibody (ABIN650724)

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling Images back to top
Product cited in:

Wu, Boonmars, Nagano, Boonjaraspinyo, Srinontong, Ratasuwan, Narong, Nielsen, Maekawa: "Significance of S100P as a biomarker in diagnosis, prognosis and therapy of opisthorchiasis-associated cholangiocarcinoma." in: International journal of cancer. Journal international du cancer, 2015

Background publications

Li, Morales, Hwang, Wagner, Boothman: "DNA mismatch repair-dependent activation of c-Abl/p73alpha/GADD45alpha-mediated apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21394-403, 2008

Al-Romaih, Sadikovic, Yoshimoto, Wang, Zielenska, Squire: "Decitabine-induced demethylation of 5' CpG island in GADD45A leads to apoptosis in osteosarcoma cells." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 5, pp. 471-80, 2008

Zhu, Ren, Korchin, Lahat, Dicker, Lu, Mills, Pollock, Lev: "Soft tissue sarcoma cells are highly sensitive to AKT blockade: a role for p53-independent up-regulation of GADD45 alpha." in: Cancer research, Vol. 68, Issue 8, pp. 2895-903, 2008

Kearsey, Coates, Prescott, Warbrick, Hall: "Gadd45 is a nuclear cell cycle regulated protein which interacts with p21Cip1." in: Oncogene, Vol. 11, Issue 9, pp. 1675-83, 1995

Images

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling References for anti-GADD45A Antibody (ABIN650724) back to top
Supplier Images
Western Blotting (WB) image for anti-GADD45A antibody (Growth Arrest and DNA-Damage-Inducible, alpha) (AA 133-165) (ABIN650724) Western blot analysis of GADD45A Antibody (C-term) (ABIN650724) in Y79 cell line lysa...
Flow Cytometry (FACS) image for anti-GADD45A antibody (Growth Arrest and DNA-Damage-Inducible, alpha) (AA 133-165) (ABIN650724) GADD45A Antibody (C-term) (ABIN650724) flow cytometric analysis of NCI-H460 cells (bo...
Western Blotting (WB) image for anti-GADD45A antibody (Growth Arrest and DNA-Damage-Inducible, alpha) (AA 133-165) (ABIN650724) Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (C-...