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anti-Human HMGB1 antibody for Immunohistochemistry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Epitope
Center
34
27
18
17
13
12
12
11
8
7
7
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
295
112
101
19
19
17
6
6
3
1
1
1
Host
Rabbit
212
94
4
4
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
20
15
11
9
8
7
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
250
158
74
59
52
27
13
12
11
6
6
2
1
1
1
1
Supplier
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Catalog No. ABIN2854997
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.21876736 ABIN250705 ICC FACS IF IHC (p) IHC WB Rabbit AA 100-200 Log in to see Polyclonal
0.21876736 ABIN2862626 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.21876736 ABIN934042 IHC ELISA WB Mouse IgG2b kappa AA 1-215 Log in to see J2E1
0.21876736 ABIN2470973 IF IHC ELISA WB Mouse IgG2a kappa AA 1-216 Log in to see 1B11
0.21876736 ABIN2470972 IF IHC ELISA WB Mouse IgG2a kappa AA 1-216 Log in to see 1D5
0.21876736 ABIN2470974 IF IHC ELISA WB Mouse IgG2a kappa AA 1-91 Log in to see 2F6
0.21876736 ABIN2439675 IHC WB Mouse IgM C-Term Log in to see 393CT23-1-4
0.21876736 ABIN2405450 IHC WB Rabbit IgG Log in to see Polyclonal
0.21876736 ABIN2426065 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.21876736 ABIN3023358 IHC WB Rabbit Log in to see Polyclonal
0.0011390075 ABIN377210 IHC ELISA WB Mouse IgG2b AA 1-215 Log in to see J2E1
0.0011390075 ABIN1873039 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011390075 ABIN1873041 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011390075 ABIN1584565 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0011390075 ABIN1735426 ICC FACS IF IHC WB Rabbit Internal Region, AA 100-200 Log in to see Polyclonal
0.0011390075 ABIN3021120 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011390075 ABIN2892072 IF IHC WB Rabbit Ala164 Log in to see Polyclonal
0.0011390075 ABIN2957616 IHC WB Rabbit N-Term Log in to see Polyclonal
0.0011390075 ABIN448273 ICC FACS IF IHC (p) IHC WB Rabbit IgG Log in to see EPR3507
0.0011390075 ABIN448285 FACS IHC (p) IHC WB Rabbit IgG Log in to see EPR3506

Similar anti-HMGB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 42 Antibodies 43 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies 14 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies 8 Antibodies
Immunofluorescence (IF) 15 Antibodies 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 27 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 76 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope Center
(34), (27), (18), (17), (13), (12), (12), (11), (8), (7), (7), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(295), (112), (101), (19), (19), (17), (6), (6), (3), (1), (1), (1)
Host Rabbit
(212), (94), (4), (4), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(20), (15), (11), (9), (8), (7), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(250), (158), (74), (59), (52), (27), (13), (12), (11), (6), (6), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Cross-Reactivity Dog (Canine), Pig (Porcine), Rabbit, Rhesus Monkey, Cow (Bovine)
Cross-Reactivity (Details) Dog (100 %), Pig (100 %), Rabbit (100 %), Rhesus Monkey (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to HMGB1 (high-mobility group box 1)
HMGB1 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
Isotype IgG

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Cellular Localization: Nucleus
Molecular Weight 25 kDa
Gene ID 3146
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Immunohistochemistry Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* ImmunohistochemistryAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration 1.92 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by imm...
Immunofluorescence (IF) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) ICC/IF Image HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent ana...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by western blot analysis. Various whole...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg NIH-...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg PC-1...
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immu...
Immunofluorescence (IF) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) ICC/IF Image HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent ana...
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunoh...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) WB Image HMGB1 antibody detects HMGB1 protein by Western blot analysis. A. 30 µg 293T...
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (Center) (ABIN2854997) IHC-P Image Immunohistochemical analysis of paraffin-embedded 59T xenograft, using HM...