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anti-Mammalian HMGB1 antibody for Western Blotting

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Reactivity
Human, Cow (Bovine), Pig (Porcine), Mouse (Murine), Rat (Rattus), Mammalian
396
183
114
29
26
21
10
9
6
6
2
1
1
Host
Mouse
229
178
4
4
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
24
17
16
14
8
7
7
6
6
6
6
6
5
5
4
4
4
4
4
4
3
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
344
178
129
86
83
61
22
12
11
11
6
6
2
1
1
1
1
Supplier
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Catalog No. ABIN1580427
$ 220.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011731815 ABIN1176834 Inhibition CA IF IHC WB Mouse IgG1 Log in to see DPH1-1 1
0.0011731815 ABIN1951825 ICC IF WB Mouse IgG2b Log in to see

Top referenced anti-HMGB1 antibody for Western Blotting

Similar anti-HMGB1 Antibodies

Application / Reactivity Human Pig (Porcine) Cow (Bovine) Mouse (Murine) Rat (Rattus) Mammalian
Western Blotting (WB) 338 Antibodies 20 Antibodies 30 Antibodies 156 Antibodies 88 Antibodies 3 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 12 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 131 Antibodies 18 Antibodies 70 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Reactivity Human, Cow (Bovine), Pig (Porcine), Mouse (Murine), Rat (Rattus), Mammalian
(396), (183), (114), (29), (26), (21), (10), (9), (6), (6), (2), (1), (1)
Host Mouse
(229), (178), (4), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is un-conjugated
(24), (17), (16), (14), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(344), (178), (129), (86), (83), (61), (22), (12), (11), (11), (6), (6), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Purification affinity purified antibody
Clone 1F3
Isotype IgG2b

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name High mobility group box 1, Amphoterin, HMGB1 (HMGB1 Antibody Abstract)
Background High-mobility group proteins were named originally since they are abundant relatively low molecular weight proteins which run quickly on SDS-PAGE gels. High-mobility group proteis box 1 (HMGB1) is one of these. The bx in the name refers to the so-called high mobility group (HMG) box, a compact domain involved in DNA binding and protein-protein interactions. The HMGB1 molecule has two of these HMG domains. The protein is also called amphoterin, this name being derived from the presence of two highly charged regions in the molecule, a relatively neutrally charged N-terminus and a very negatively charged C-terminus. In fact, the molecule is very unusually charged throughout, the human sequence consisting of 16.7% Glutamic acid, 9.3% Aspartic acid, 20% Lysine and 9.3% Arginine. HMGB1 can bind Toll like receptor 4 (TLR4) and the Receptor for Advanced Glycation End products (RAGE). TLRs are components of the innate immune system, first recognized as a family of receptors which recognize Pathogen Associated Molecular Pattern molecules (PAMPs). PAMPs are common components of bacteria and when TLRs bind these, a strong inflammatory response is activated. More recently it has been recognized that TLRs can also be activated by Damage Associated Molecular Pattern molecules (DAMPs), which are endogenous substances released from damaged and diseased cells which also bind to TLR family receptors and also activate inflammation. HMGB1 is such a DAMP, binding to TLR4, and much evidence suggests that HMGB1 is a strong activator of inflammation. Interestingly, HMGB1 is released by necrotic cells but not by apoptotic cells. The HGNC name for this protein is HBGB1.
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes The antibody solution can be used at dilutions of 1:1,000 or higher in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000-2,000.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 4°C short term or -20°C long term.

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN1580427) Blot of crude HeLa cell extract stained with MCS-1F3. HMGB1 runs at an apparent molec...
Immunofluorescence (IF) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN1580427) HeLa cells grown in tissue culture and stained with ABIN1580427 (red), chicken polycl...