anti-Human FOXO1 antibody for Proximity Ligation Assay

Recommended FOXO1 Antibody (supplied by: Log in to see )

Antigen
Forkhead Box O1 (FOXO1) Antibodies
  • FOXO1
  • FOXO1A
  • fkh1
  • fkhr
  • foxo1a
  • xFoxO1
  • FKH1
  • FKHR
  • AI876417
  • Afxh
  • Fkhr1
  • Foxo1a
  • Fkhr
  • FoxO1A
  • FoxO1a.1
  • foxo1
  • zgc:153388
  • forkhead box O1
  • forkhead box O1 a
  • FOXO1
  • foxo1
  • Foxo1
  • foxo1a
Epitope
C-Term
54
36
32
27
22
19
18
16
16
10
10
9
8
8
5
5
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
470
254
202
35
29
21
14
12
8
7
5
3
3
3
3
2
2
1
1
Host
Rabbit
320
134
21
2
Clonality
Polyclonal
Conjugate
This FOXO1 antibody is un-conjugated
11
11
9
8
8
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
381
206
135
133
131
82
56
11
9
9
5
4
4
3
3
3
2
1
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Catalog No. ABIN250693
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
32.20224 ABIN560917 IF PLA ELISA WB Mouse IgG2a kappa AA 556-655, partial Log in to see 4E9
32.20224 ABIN560919 IF PLA ELISA WB Mouse IgG2a kappa AA 556-655, partial Log in to see 4B4
1 ABIN1896479 IHC IP PLA WB Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen Forkhead Box O1 (FOXO1) Antibodies
Epitope C-Term
(54), (36), (32), (27), (22), (19), (18), (16), (16), (10), (10), (9), (8), (8), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(470), (254), (202), (35), (29), (21), (14), (12), (8), (7), (5), (3), (3), (3), (3), (2), (2), (1), (1)
Host Rabbit
(320), (134), (21), (2)
Clonality
Polyclonal
Conjugate This FOXO1 antibody is un-conjugated
(11), (11), (9), (8), (8), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(381), (206), (135), (133), (131), (82), (56), (11), (9), (9), (5), (4), (4), (3), (3), (3), (2), (1)
Pubmed 8 references available
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Product Details anti-FOXO1 Antibody

Target Details FOXO1 Application Details Handling References for anti-FOXO1 antibody (ABIN250693) Images
Purification Immunogen affinity purified
Immunogen The immunogen recognized by this antibody maps to a region between residues 600 and the C-terminus (residue 652) of human Forkhead Box O1a using the numbering given in Swiss-Prot entry Q12778 (GeneID 2308).
Isotype IgG

Target Details FOXO1

Product Details anti-FOXO1 Antibody Application Details Handling References for anti-FOXO1 antibody (ABIN250693) Images back to top
Antigen
Alternative Name FoxO1/FKHR (FOXO1 Antibody Abstract)
Background Gene Symbol: FOXO1
Gene ID 2308
UniProt Q12778
Pathways PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events

Application Details

Product Details anti-FOXO1 Antibody Target Details FOXO1 Handling References for anti-FOXO1 antibody (ABIN250693) Images back to top
Application Notes Western Blot 1:3000-1:10000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1-10 μg/mg lysate, Immunohistochemistry-Paraffin, Proximity Ligation Assay 1:1000-1:10000Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25344740)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-FOXO1 Antibody Target Details FOXO1 Application Details References for anti-FOXO1 antibody (ABIN250693) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-FOXO1 antibody (ABIN250693)

Product Details anti-FOXO1 Antibody Target Details FOXO1 Application Details Handling Images back to top
Product cited in:

Calvier, Chouvarine, Legchenko, Hoffmann, Geldner, Borchert, Jonigk, Mozes, Hansmann: "PPARγ Links BMP2 and TGFβ1 Pathways in Vascular Smooth Muscle Cells, Regulating Cell Proliferation and Glucose Metabolism." in: Cell metabolism, Vol. 25, Issue 5, pp. 1118-1134.e7, 2017 (Sample species: Mouse (Murine)).

Savai, Al-Tamari, Sedding, Kojonazarov, Muecke, Teske, Capecchi, Weissmann, Grimminger, Seeger, Schermuly, Pullamsetti: "Pro-proliferative and inflammatory signaling converge on FoxO1 transcription factor in pulmonary hypertension." in: Nature medicine, Vol. 20, Issue 11, pp. 1289-300, 2014 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Yin, Pavone, Lu, Wei, Kim: "Increased activation of the PI3K/AKT pathway compromises decidualization of stromal cells from endometriosis." in: The Journal of clinical endocrinology and metabolism, Vol. 97, Issue 1, pp. E35-43, 2012

van der Horst, Wang, Vandenput, Kühne, Ewing, van Ijcken, van der Zee, Amant, Burger, Blok: "Progesterone inhibits epithelial-to-mesenchymal transition in endometrial cancer." in: PLoS ONE, Vol. 7, Issue 1, pp. e30840, 2012

Amin, Thakur, Gupta, Jackson, Harada, Agarwal, Shin, Wald, Agarwal: "Restoration of p53 functions protects cells from concanavalin A-induced apoptosis." in: Molecular cancer therapeutics, Vol. 9, Issue 2, pp. 471-9, 2010

Khatri, Yepiskoposyan, Gallo, Tandon, Plas: "FOXO3a regulates glycolysis via transcriptional control of tumor suppressor TSC1." in: The Journal of biological chemistry, Vol. 285, Issue 21, pp. 15960-5, 2010

Hoekstra, Sefton, Berry, Lu, Hardt, Marsh, Yin, Clardy, Chakravarti, Bulun, Kim: "Progestins activate the AKT pathway in leiomyoma cells and promote survival." in: The Journal of clinical endocrinology and metabolism, Vol. 94, Issue 5, pp. 1768-74, 2009

Ward, Hoekstra, Blok, Hanifi-Moghaddam, Lurain, Singh, Buttin, Schink, Kim: "The regulation and function of the forkhead transcription factor, Forkhead box O1, is dependent on the progesterone receptor in endometrial carcinoma." in: Endocrinology, Vol. 149, Issue 4, pp. 1942-50, 2008

Images

Product Details anti-FOXO1 Antibody Target Details FOXO1 Application Details Handling References for anti-FOXO1 antibody (ABIN250693) back to top
Supplier Images
Western Blotting (WB) image for anti-Forkhead Box O1 (FOXO1) (C-Term) antibody (ABIN250693) Western Blot and Immunoprecipitation: FOXO1a Antibody [ABIN250693] - Detection of Hum...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Forkhead Box O1 (FOXO1) (C-Term) antibody (ABIN250693) Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [ABIN250693] - Sample: FFPE sectio...
Proximity Ligation Assay (PLA) image for anti-Forkhead Box O1 (FOXO1) (C-Term) antibody (ABIN250693) Proximity Ligation Assay: FoxO1/FKHR Antibody [ABIN250693] - Secondary-conjugate Duol...
Immunocytochemistry (ICC) image for anti-Forkhead Box O1 (FOXO1) (C-Term) antibody (ABIN250693) Immunohistochemistry: FoxO1/FKHR Antibody - Sample: FFPE section of human ovarian ca...
Western Blotting (WB) image for anti-Forkhead Box O1 (FOXO1) (C-Term) antibody (ABIN250693) Western Blot: FoxO1/FKHR Antibody - Whole cell lysate from 293T, HeLa, and Jurkat ce...
Western Blotting (WB) image for anti-Forkhead Box O1 (FOXO1) (C-Term) antibody (ABIN250693) Western Blot: FoxO1/FKHR Antibody - Detection of Human FOXO1a by Western Blot. Sampl...