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anti-Dog (Canine) MTOR antibody for Immunofluorescence

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
AA 2440-2457
62
28
26
22
21
21
15
15
11
10
9
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
351
198
196
18
8
8
7
7
7
7
4
2
2
1
1
1
Host
Rabbit
300
47
3
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
7
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
232
110
91
65
53
52
37
19
7
7
7
6
3
2
2
2
1
1
1
Supplier
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Available images

Catalog No. ABIN303334
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1915272 ICC IF IP PLA Rabbit IgG AA 200-250 Log in to see Polyclonal

Similar anti-MTOR Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Zebrafish (Danio rerio)
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies 4 Antibodies 4 Antibodies
Western Blotting (WB) 234 Antibodies 110 Antibodies 106 Antibodies 6 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 2 Antibodies 1 Antibodies 1 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope AA 2440-2457
(62), (28), (26), (22), (21), (21), (15), (15), (11), (10), (9), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
(351), (198), (196), (18), (8), (8), (7), (7), (7), (7), (4), (2), (2), (1), (1), (1)
Host Rabbit
(300), (47), (3), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(232), (110), (91), (65), (53), (52), (37), (19), (7), (7), (7), (6), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling Images
Specificity This antibody recognises MTOR.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog) and Zebrafish.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography
Immunogen Synthetic Peptide corresponding to Amino acids 2440-2457 of human mTOR.

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling Images back to top
Antigen
Alternative Name mTOR / FRAP1 (FRAP1 Antibody Abstract)
Background The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin. Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. TOR is normally associated with the regulatory proteins RAPTOR and GbL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling Images back to top
Application Notes Immunohistochemistry on Paraffin Sections (2.5 μg/mL). Immunofluorescence. Western Blot (1/250-1/2000). ELISA (1/4000-1/20000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details Images back to top
Concentration 0,6 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling back to top
Supplier Images
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN303334) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN303334) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody (Image 2)