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anti-Human MTOR antibody for Immunoprecipitation

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
C-Term
62
26
26
21
21
20
15
15
12
10
9
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
346
196
194
18
9
8
7
7
7
7
4
2
2
2
1
1
Host
Rabbit
297
45
3
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
7
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
227
108
89
65
53
52
36
16
8
7
7
4
3
2
2
2
1
1
Options
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Catalog No. ABIN2855088
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Log in to see Polyclonal 4
1 ABIN2666241 IF ICFC CyTOF IP WB Rabbit Ig Fraction pSer2481 Log in to see Polyclonal 6
1 ABIN1868039 ICC IHC IP WB Rabbit IgG AA 2226-2488 Log in to see Polyclonal
1 ABIN2755575 IC IF IP IHC WB Rabbit C-Term, pSer2448 Log in to see Polyclonal
1 ABIN153335 IP Agarose Beads Rabbit AA 200-250 Log in to see Polyclonal
1 ABIN1822694 IF IP WB Rabbit IgG Ser2448 Log in to see
1 ABIN601930 IHC (p) IP WB Rabbit IgG AA 1223-1290 Log in to see Polyclonal
1 ABIN1737125 IP Rabbit IgG Log in to see Polyclonal
1 ABIN1737126 IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN220569 IP ELISA WB Rabbit IgG Ser2481 Log in to see Polyclonal
1 ABIN220571 IP ELISA WB Rabbit IgG pSer2448 Log in to see Polyclonal
1 ABIN2308337 IF IP WB Rabbit IgG pSer2448 Log in to see 11C1050
1 ABIN2308330 IP IHC WB Rabbit IgG AA 1223-1290 Log in to see Polyclonal
1 ABIN153336 IP Agarose Beads Rabbit AA 200-250 Log in to see Polyclonal
1 ABIN190048 IP WB Rabbit Log in to see Polyclonal
1 ABIN190047 IP WB Rabbit Log in to see Polyclonal
1 ABIN1110154 ICC IF IP Rabbit IgG Exon 5, Exon 6 Log in to see Polyclonal
1 ABIN328657 ICC IP WB Rabbit AA 200-250 Log in to see Polyclonal
1 ABIN341836 IP Rabbit Log in to see Polyclonal
1 ABIN445054 IP Goat IgG Log in to see Polyclonal

Top referenced anti-MTOR antibody for Immunoprecipitation

Similar anti-MTOR Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies 1 Antibodies
Electron Microscopy (EM) 1 Antibodies
ELISA 38 Antibodies 40 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 4 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 34 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 43 Antibodies
Immunoprecipitation (IP) 27 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 104 Antibodies
Flow Cytometry (FACS) 1 Antibodies

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope C-Term
(62), (26), (26), (21), (21), (20), (15), (15), (12), (10), (9), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(346), (196), (194), (18), (9), (8), (7), (7), (7), (7), (4), (2), (2), (2), (1), (1)
Host Rabbit
(297), (45), (3), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(227), (108), (89), (65), (53), (52), (36), (16), (8), (7), (7), (4), (3), (2), (2), (2), (1), (1)
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))
mTOR antibody [C3], C-term
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.
Isotype IgG

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling Images back to top
Antigen
Alternative Name mTOR (FRAP1 Antibody Abstract)
Background The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Cellular Localization: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side , Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side , Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side
Molecular Weight 289 kDa
Gene ID 2475
Research Area Cardiovascular, Cardiogenesis, Cell Cycle, DNA/RNA
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling Images back to top
Application Notes Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) ICC/IF Image mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunoflu...
Immunoprecipitation (IP) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) IP Image Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg...
Immunohistochemistry (IHC) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse ...
Immunohistochemistry (IHC) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) IHC-P Image mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immun...
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) WB Image mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue ex...
Immunoprecipitation (IP) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) IP Image mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: ...
Chromatin Immunoprecipitation (ChIP) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) ChIP Image Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of ei...
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) WB Image mTOR antibody detects mTOR protein by western blot analysis. Various whole c...
Immunohistochemistry (IHC) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (C-Term) (ABIN2855088) IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse tes...