anti-Mouse (Murine) MTOR antibody for Western Blotting

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
pThr2446
66
36
27
23
21
15
15
13
12
10
8
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
374
198
196
18
9
8
7
7
7
7
4
2
2
2
1
1
Host
Rabbit
322
48
3
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
8
7
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
ELISA, Western Blotting (WB)
250
113
97
73
56
52
37
20
8
7
7
6
3
2
2
2
1
1
1
Options
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Catalog No. ABIN1531715
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.90624 ABIN1531910 IHC ELISA WB Rabbit IgG pSer2448 Log in to see Polyclonal 1
2.90624 ABIN1532744 ELISA WB Rabbit IgG Log in to see Polyclonal 3
2.90624 ABIN968383 IF WB Mouse IgG1 AA 185-290 Log in to see 30-RAFT1 5
2.90624 ABIN968384 IF WB Mouse IgG1 AA 185-290 Log in to see 30-RAFT1 5
2.90624 ABIN447030 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
2.90624 ABIN2855089 WB Rabbit IgG Internal Region Log in to see Polyclonal
2.90624 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Log in to see Polyclonal
2.90624 ABIN3182280 ELISA WB Rabbit IgG pThr2446 Log in to see Polyclonal
2.90624 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Log in to see Polyclonal
2.90624 ABIN3185708 ELISA IHC WB Rabbit IgG Ser394 Log in to see Polyclonal
2.90624 ABIN3185707 ELISA IHC WB Rabbit IgG Ser392 Log in to see Polyclonal
2.90624 ABIN1532942 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.90624 ABIN1532464 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.90624 ABIN2957676 WB Rabbit C-Term, pSer2446 Log in to see Polyclonal
2.90624 ABIN2705013 IC IF WB Rabbit C-Term Log in to see Polyclonal
2.90624 ABIN498698 IHC (p) WB Rabbit pSer2448 Log in to see Polyclonal
2.90624 ABIN2855088 ChIP ICC IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
2.90624 ABIN498610 IF IHC (p) WB Rabbit Log in to see Polyclonal
2.90624 ABIN2737201 WB Rabbit IgG Log in to see Polyclonal
2.90624 ABIN307079 WB Mouse IgG1 Log in to see 215Q18 6

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope pThr2446
(66), (36), (27), (23), (21), (15), (15), (13), (12), (10), (8), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(374), (198), (196), (18), (9), (8), (7), (7), (7), (7), (4), (2), (2), (2), (1), (1)
Host Rabbit
(322), (48), (3), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application ELISA, Western Blotting (WB)
(250), (113), (97), (73), (56), (52), (37), (20), (8), (7), (7), (6), (3), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling ProductDetails: References for anti-MTOR antibody (ABIN1531715) Images
Specificity MTOR (Phospho-Thr2446) Antibody detects endogenous levels of mTOR only when phosphorylated at Thr2446.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Thr2446.
Isotype IgG

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling ProductDetails: References for anti-MTOR antibody (ABIN1531715) Images back to top
Antigen
Alternative Name mTOR (FRAP1 Antibody Abstract)
Background Molecular weight of target protein: 288 kDa
Synonyms: FRAP, FRAP1, FRAP2, RAFT1, Rapamycin target protein, kinase mTOR
Molecular Weight 288 kDa
Gene ID 2475
OMIM 601231
UniProt P42345
Research Area Cardiovascular, Cardiogenesis, Cell Cycle, DNA/RNA
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling ProductDetails: References for anti-MTOR antibody (ABIN1531715) Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:10000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.338207 (NCBI Gene Symbol: MTOR)

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details ProductDetails: References for anti-MTOR antibody (ABIN1531715) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-MTOR antibody (ABIN1531715)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Background publications

Gregory, Barlow, McLay, Kaul, Swarbreck, Dunham, Scott, Howe, Woodfine, Spencer, Jones, Gillson, Searle, Zhou, Kokocinski, McDonald, Evans, Phillips, Atkinson, Cooper, Jones, Hall, Andrews, Lloyd et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006

Onyango, Lubyova, Gardellin, Kurzbauer, Weith: "Molecular cloning and expression analysis of five novel genes in chromosome 1p36." in: Genomics, Vol. 50, Issue 2, pp. 187-98, 1998

Brown, Albers, Shin, Ichikawa, Keith, Lane, Schreiber: "A mammalian protein targeted by G1-arresting rapamycin-receptor complex." in: Nature, Vol. 369, Issue 6483, pp. 756-8, 1994

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling ProductDetails: References for anti-MTOR antibody (ABIN1531715) back to top
Supplier Images
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pThr2446) antibody (ABIN1531715) Western blot analysis of extracts from NIH-3T3 cells treated with Insulin 0.01U/ml 15...