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|Antigen||Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, gamma (PIP5K1C) Antibodies|
|Conjugate||This PIP5K1C antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
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Product Details anti-PIP5K1C AntibodyTarget Details PIP5K1C Application Details Handling References for anti-PIP5K1C antibody (ABIN360480) Images
|Specificity||This antibody reacts to PIP5Ki gamma.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PIP5K1G.|
Target Details PIP5K1CProduct Details anti-PIP5K1C Antibody Application Details Handling References for anti-PIP5K1C antibody (ABIN360480) Images back to top
|Alternative Name||PIP5K1C (PIP5K1C Antibody Abstract)|
|Background||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.Synonyms: KIAA0589, PIP5K1-gamma, PIP5KIgamma, Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma, PtdIns(4)P-5-kinase 1 gamma|
|Gene ID||23396, 9606|
|Pathways||PI3K-Akt Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization, Maintenance of Protein Location, Synaptic Vesicle Exocytosis|
Application DetailsProduct Details anti-PIP5K1C Antibody Target Details PIP5K1C Handling References for anti-PIP5K1C antibody (ABIN360480) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIP5K1C Antibody Target Details PIP5K1C Application Details References for anti-PIP5K1C antibody (ABIN360480) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PIP5K1C antibody (ABIN360480)Product Details anti-PIP5K1C Antibody Target Details PIP5K1C Application Details Handling Images back to top
Ling, Doughman, Firestone, Bunce, Anderson: "Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions." in: Nature, Vol. 420, Issue 6911, pp. 89-93, 2002
Di Paolo, Pellegrini, Letinic, Cestra, Zoncu, Voronov, Chang, Guo, Wenk, De Camilli: "Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin." in: Nature, Vol. 420, Issue 6911, pp. 85-9, 2002
Ishihara, Shibasaki, Kizuki, Wada, Yazaki, Asano, Oka: "Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8741-8, 1998
ImagesProduct Details anti-PIP5K1C Antibody Target Details PIP5K1C Application Details Handling References for anti-PIP5K1C antibody (ABIN360480) back to top