anti-Human RPS6KB1 antibody for Enzyme Immunoassay

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • p70S6K
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • ribosomal protein S6 kinase, polypeptide 1
  • RPS6KB1
  • Rps6kb1
Epitope
pSer418
101
32
27
26
23
23
20
20
20
19
18
18
17
16
15
15
15
14
14
10
10
8
7
6
6
6
5
5
5
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
665
446
423
73
53
41
34
33
21
13
13
8
5
5
3
3
1
Host
Rabbit
593
52
23
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
14
13
13
9
9
9
9
9
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
509
265
244
140
116
104
64
36
21
20
17
11
5
1
1
1
1
Supplier
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Catalog No. ABIN358318
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1108904 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5G9 2
1 ABIN5552808 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal
1 ABIN5551331 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN401355 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN303144 EIA IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-RPS6KB1 antibody for Enzyme Immunoassay

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 20 Antibodies
ELISA 243 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 36 Antibodies
Immunofluorescence (IF) 140 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 104 Antibodies
Immunohistochemistry (IHC) 260 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 116 Antibodies
Immunoprecipitation (IP) 63 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Simple Western (SimWes) 1 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope pSer418
(101), (32), (27), (26), (23), (23), (20), (20), (20), (19), (18), (18), (17), (16), (15), (15), (15), (14), (14), (10), (10), (8), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(665), (446), (423), (73), (53), (41), (34), (33), (21), (13), (13), (8), (5), (5), (3), (3), (1)
Host Rabbit
(593), (52), (23), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (14), (13), (13), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(509), (265), (244), (140), (116), (104), (64), (36), (21), (20), (17), (11), (5), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN358318) Images
Specificity This antibody detects RPS6KB1 pSer418. Predicted to cross react with Mouse and Rat (100 % Antigen Homology).
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser418 of human RPS6KB1.
Isotype Ig

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN358318) Images back to top
Antigen
Alternative Name RPS6KB1 / STK14A (RPS6KB1 Antibody Abstract)
Background This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1
Molecular Weight 59009 Da
Gene ID 6198, 9606
UniProt P23443
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Smooth Muscle Cell Migration

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN358318) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details ProductDetails: References for anti-RPS6KB1 antibody (ABIN358318) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-RPS6KB1 antibody (ABIN358318)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Background publications

Adem, Soderberg, Hafner, Reynolds, Slezak, Sinclair, Sellers, Schaid, Couch, Hartmann, Jenkins: "ERBB2, TBX2, RPS6KB1, and MYC alterations in breast tissues of BRCA1 and BRCA2 mutation carriers." in: Genes, chromosomes & cancer, Vol. 41, Issue 1, pp. 1-11, 2004

Suzuki, Yamashita, Shirota, Sakakibara, Chiba, Mizushima-Sugano, Nakai, Sugano: "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions." in: Genome research, Vol. 14, Issue 9, pp. 1711-8, 2004

Gomez-Cambronero, Frye, Baumann: "Ribosomal p70S6K basal activity increases upon induction of differentiation of myelomonocytic leukemic cell lines HL60, AML14 and MPD." in: Leukemia research, Vol. 28, Issue 7, pp. 755-62, 2004

Raught, Peiretti, Gingras, Livingstone, Shahbazian, Mayeur, Polakiewicz, Sonenberg, Hershey: "Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases." in: The EMBO journal, Vol. 23, Issue 8, pp. 1761-9, 2004

Miyakawa, Tsushima, Murakami, Wakai, Isozaki, Takano: "Increased expression of phosphorylated p70S6 kinase and Akt in papillary thyroid cancer tissues." in: Endocrine journal, Vol. 50, Issue 1, pp. 77-83, 2003

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling ProductDetails: References for anti-RPS6KB1 antibody (ABIN358318) back to top
Supplier Images
 image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (pSer418) (ABIN358318) anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (pSer418) antibody