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anti-Human RPS6KB1 antibody for ELISA

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • 70kDa
  • 2610318I15Rik
  • 4732464A07Rik
  • AA959758
  • AI256796
  • AI314060
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • p70/85s6k
  • p70S6K
  • p70s6k
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
Reactivity
Human
649
360
345
48
30
21
16
15
6
1
1
1
1
Host
Mouse
592
39
25
2
Clonality
Monoclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
13
13
13
8
8
8
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
497
282
250
148
93
88
59
50
19
11
9
9
1
1
1
Supplier
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Catalog No. ABIN969565
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30628014 ABIN1327091 ELISA WB Mouse IgG2b kappa AA 416-525, partial Log in to see 1E24
0.30628014 ABIN562721 IF IHC (p) ELISA WB Mouse IgG2b kappa AA 416-525, partial Log in to see 1E12
0.30628014 ABIN562722 IF PLA ELISA WB Mouse IgG IgA IgM Mix AA 416-525, partial Log in to see 1E10
0.30628014 ABIN519894 IF ELISA WB Mouse IgG1 kappa AA 416-525, partial Log in to see 2C2
0.30628014 ABIN519895 IF ELISA WB Mouse IgG1 kappa AA 416-525, partial Log in to see 4H4
0.30628014 ABIN1846066 FACS IHC ELISA WB Mouse IgG1 Log in to see
0.0012155735 ABIN1531360 IF IHC ELISA WB Rabbit IgG pThr389 Log in to see Polyclonal 2
0.0012155735 ABIN1533663 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012155735 ABIN1533098 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012155735 ABIN184828 IHC ELISA WB Goat C-Term Log in to see Polyclonal 1
0.0012155735 ABIN361700 ICC IF IP ELISA WB Rabbit C-Term Log in to see Polyclonal 6
0.0012155735 ABIN2486867 ICC IF IP ELISA WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486868 ICC IF IP ELISA WB Atto 488 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486869 ICC IF IP ELISA WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486870 ICC IF IP ELISA WB Atto 594 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486871 ICC IF IP ELISA WB Atto 633 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486872 ICC IF IP ELISA WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486874 ICC IF IP ELISA WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486873 ICC IF IP ELISA WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486875 ICC IF IP ELISA WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 3

Top referenced anti-RPS6KB1 antibody for ELISA

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
ELISA 250 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 50 Antibodies
Immunofluorescence (IF) 148 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 88 Antibodies
Immunohistochemistry (IHC) 279 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 93 Antibodies
Immunoprecipitation (IP) 57 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 490 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Reactivity Human
(649), (360), (345), (48), (30), (21), (16), (15), (6), (1), (1), (1), (1)
Host Mouse
(592), (39), (25), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (13), (13), (13), (8), (8), (8), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(497), (282), (250), (148), (93), (88), (59), (50), (19), (11), (9), (9), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN969565) Images
Purification Purified
Immunogen Purified recombinant fragment of human RPS6KB1 expressed in E. coli.
Clone 5G9
Isotype IgG1

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 Antibody (ABIN969565) Images back to top
Antigen
Alternative Name RPS6KB1 (RPS6KB1 Antibody Abstract)
Background This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
Molecular Weight 59 kDa
Gene ID 6198
HGNC 6198
Research Area Signaling, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 Antibody (ABIN969565) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 Antibody (ABIN969565) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-RPS6KB1 Antibody (ABIN969565)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Product cited in:

Jan, Adolfsson, Allaman, Buccarello, Magistretti, Pfeifer, Muhs, Lashuel: "Abeta42 neurotoxicity is mediated by ongoing nucleated polymerization process rather than by discrete Abeta42 species." in: The Journal of biological chemistry, Vol. 286, Issue 10, pp. 8585-96, 2011

Deshmukh, Salehzadeh, Metayer-Coustard, Fahlman, Nair, Al-Khalili: "Post-transcriptional gene silencing of ribosomal protein S6 kinase 1 restores insulin action in leucine-treated skeletal muscle." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 8, pp. 1457-66, 2009

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN969565) back to top
Supplier Images
ELISA image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 2: Flow cytometric analysis of Jurkat cells using RPS6KB1 mouse mAb (green) an...
Immunohistochemistry (IHC) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues usi...
Immunohistochemistry (IHC) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 4: Immunohistochemical analysis of paraffin-embedded muscle tissues using RPS6...
Western Blotting (WB) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 5: Western blot analysis using RPS6KB1 mAb against human RPS6KB1 (AA: 295-524)...