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Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 2-232), (partial) protein (GST tag)

Details for Product No. ABIN1046521
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Protein Name
Protein Characteristics
AA 2-232, partial
Origin
Human
(7), (1)
Source
Escherichia coli (E. coli)
(4), (2), (2)
Protein Type Recombinant
Purification tag / Conjugate
GST tag
Application
SDS-PAGE (SDS), ELISA
Pubmed 5 references available
Quantity 10 µg
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Catalog No. ABIN1046521
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Sequence ASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLL NEMSRTGEGAVSLMERRRLNQLLLPLLQGPDITLSKLYKL IEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSG TEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQY FQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEV SCSGPLSQKQAEFFLSQQASLLKNDETKALT
Characteristics Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Ref.7
Purity 90 %
Alternative Name Anaphase-promoting complex subunit 5
Background Synonyms: APC5,Cyclosome subunit 5
Molecular Weight 53.6 kD
UniProt Q9UJX4
Comment

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Restrictions For Research Use only
Buffer PBS buffer, 20mM GSH
Storage -20 °C
Product cited in: Yu, Peters, King et al.: "Identification of a cullin homology region in a subunit of the anaphase-promoting complex." in: Science (New York, N.Y.), Vol. 279, Issue 5354, pp. 1219-22, 1998 (PubMed).

Kraft, Herzog, Gieffers et al.: "Mitotic regulation of the human anaphase-promoting complex by phosphorylation." in: The EMBO journal, Vol. 22, Issue 24, pp. 6598-609, 2003 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Bechtel, Rosenfelder, Duda et al.: "The full-ORF clone resource of the German cDNA Consortium." in: BMC genomics, Vol. 8, pp. 399, 2008 (PubMed).

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