GFRA2 Protein (AA 22-441) (His tag)

Catalog No. ABIN1096735

Product Details

Target: GFRA2 (GDNF Family Receptor alpha 2 (GFRA2))

Protein Type: Recombinant

Protein Characteristics: AA 22-441

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This GFRA2 protein is labelled with His tag.

Purpose: Recombinant Human GDNF Receptor α-2/GFRA2 (C-6His)

Sequence: SPSSLQGPEL HGWRPPVDCV RANELCAAES NCSSRYRTLR QCLAGRDRNT MLANKECQAA LEVLQESPLY DCRCKRGMKK ELQCLQIYWS IHLGLTEGEE FYEASPYEPV TSRLSDIFRL ASIFSGTGAD PVVSAKSNHC LDAAKACNLN DNCKKLRSSY ISICNREISP TERCNRRKCH KALRQFFDRV PSEYTYRMLF CSCQDQACAE RRRQTILPSC SYEDKEKPNC LDLRGVCRTD HLCRSRLADF HANCRASYQT VTSCPADNYQ ACLGSYAGMI GFDMTPNYVD SSPTGIVVSP WCSCRGSGNM EEECEKFLRD FTENPCLRNA IQAFGNGTDV NVSPKGPSFQ ATQAPRVEKT PSLPDDLSDS TSLGTSVITT CTSVQEQGLK ANNSKELSMC FTELTTNIIP GSNKVIKPNS VDHHHHHH

Characteristics: Recombinant Human GDNF Family Receptor alpha-2/GFRA2 produced by transfected human cells is a secreted protein with sequence (Ser22-Ser441) of Human GFRA2 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: GFRA2 (GDNF Family Receptor alpha 2 (GFRA2))

Alternative Name: gfr-alpha-2 (GFRA2 Products)

Synonyms: GDNFRB Protein, NRTNR-ALPHA Protein, NTNRA Protein, RETL2 Protein, TRNR2 Protein, Retl2 Protein, NTNRALPHA Protein, ntnra Protein, retl2 Protein, trnr2 Protein, gdnfrb Protein, nrtnr-alpha Protein, gfra2 Protein, GDNF family receptor alpha 2 Protein, glial cell line derived neurotrophic factor family receptor alpha 2 Protein, GDNF family receptor alpha 2a Protein, GFRA2 Protein, Gfra2 Protein, gfra2 Protein, gfra2a Protein

Sub Type: Fusionprotein

Background: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and Neurturin, play key roles in the control of vertebrate neuronal survivial and differentiation. GDNF is a glycosylated, disulfide-bonded homodimer that is distantly related to the TGF superfamily of growth factors. Three receptors for these factors, GFRalpha-1, GFRalpha-2, and GFRalpha-3 have been identified. The receptors do not contain transmembrane domains and are attached to the cell membrane by glycosyl-phosphoinositol linkage. Both GFRalpha-1 and GFRalpha-2 have been shown to mediate the GDNF-dependent and Neurturin-dependent phosphorylation and activation of the tyrosine kinase Ret. GFR-3 is expressed only during development. GFRalpha-2 binds Neurturin and mediates activation of RET receptor tyrosine kinase by both Neurturin and GDNF.
Alternative Names: GDNF Family Receptor Alpha-2, GDNF Receptor Alpha-2, GDNFR-Alpha-2, GFR-Alpha-2, GDNF Receptor Beta, GDNFR-Beta, Neurturin Receptor Alpha, NRTNR-Alpha, NTNR-Alpha, RET Ligand 2, TGF-Beta-Related Neurotrophic Factor Receptor 2, GFRA2, GDNFRB, RETL2, TRNR2

Molecular Weight: 47.79 kDa

UniProt: O00451