HSF2 Protein (AA 411-536) (His tag)

Catalog No. ABIN1096839

Product Details

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Protein Type: Recombinant

Protein Characteristics: AA 411-536

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This HSF2 protein is labelled with His tag.

Purpose: Recombinant Human Heat Shock Factor Protein 2/HSF2 (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MSENKGLETT KNNVVQPVSE EGRKSKSKPD KQLIQYTAFP LLAFLDGNPA SSVEQASTTA SSEVLSSVDK PIEVDELLDS SLDPEPTQSK LVRLEPLTEA EASEATLFYL CELAPAPLDS DMPLLDS

Characteristics: Recombinant Human Heat Shock Factor Protein 2/HSF2 is produced by our E. coli expression system. The target protein is expressed with sequence (Ser411-Ser536) of Human HSF2 fused with a 6His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.2.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Alternative Name: Heat Shock Factor Protein 2/HSF2 (HSF2 Products)

Synonyms: AI661205 Protein, HSF 2 Protein, HSTF 2 Protein, HSF3B Protein, HSTF3B Protein, heat shock factor 2 Protein, heat shock transcription factor 2 Protein, Hsf2 Protein, HSF2 Protein

Sub Type: Fusionprotein

Background: Heat Shock Factor Protein 2 (HSF2) belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is widely expressed in many cells and tissues. HSF2 is located on Cytoplasmic during normal growth. But when it is activited, HSF2 moves to the nucleus.
Alternative Names: Heat Shock Factor Protein 2, HSF 2, Heat Shock Transcription Factor 2, HSTF 2, HSF2, HSTF2

Molecular Weight: 15.9 kDa

UniProt: Q03933