TWSG1 Protein (AA 26-223) (His tag)

Catalog No. ABIN1097715

Product Details

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Protein Type: Recombinant

Protein Characteristics: AA 26-223

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This TWSG1 protein is labelled with His tag.

Purpose: Recombinant Human TWSG1/TSG (C-6His)

Sequence: CNKALCASDV SKCLIQELCQ CRPGEGNCSC CKECMLCLGA LWDECCDCVG MCNPRNYSDT PPTSKSTVEE LHEPIPSLFR ALTEGDTQLN WNIVSFPVAE ELSHHENLVS FLETVNQPHH QNVSVPSNNV HAPYSSDKEH MCTVVYFDDC MSIHQCKISC ESMGASKYRW FHNACCECIG PECIDYGSKT VKCMNCMFVD HHHHHH

Characteristics: Recombinant Human Twisted Gastrulation Protein Homolog 1/TWSG1 produced by transfected human cells is a secreted protein with sequence (Cys26 -Phe223) of human TWSG1 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Alternative Name: tsg (TWSG1 Products)

Synonyms: TWSG1 Protein, TSG Protein, DKFZp469J051 Protein, 1810013J15Rik Protein, 9030422N06Rik Protein, AW552143 Protein, D17Ertd403e Protein, Tsg Protein, Twg Protein, fi23f01 Protein, tsg Protein, tsga Protein, twsg1 Protein, wu:fi23f01 Protein, twsg Protein, tsg1 Protein, tsgb Protein, ztsg1 Protein, twisted gastrulation BMP signaling modulator 1 Protein, twisted gastrulation homolog 1 (Drosophila) Protein, twisted gastrulation BMP signaling modulator 1a Protein, twisted gastrulation BMP signaling modulator 1 S homeolog Protein, twisted gastrulation BMP signaling modulator 1 L homeolog Protein, twisted gastrulation BMP signaling modulator 1b Protein, TWSG1 Protein, Twsg1 Protein, twsg1a Protein, twsg1.S Protein, twsg1.L Protein, twsg1b Protein

Sub Type: Fusionprotein

Background: Twisted Gastrulation Protein Homolog 1 (TWSG1) is a 22 kDa secreted protein that belongs to the twisted gastrulation protein family. Human TWSG1 is synthesized as a 223 aa precursor that contains a 25 aa signal peptide and a 198 aa mature chain. TWSG1 may be involved in dorsoventral axis formation. TWSG1 seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors.TWSG1 can inhibit BMP activity by binding directly to BMP proteins, and can act the anti-BMP function, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. TWSG1 may be an important modulator of BMP-regulated cartilage development, chondrocyte differentiation and thymocyte development.
Alternative Names: Twisted Gastrulation Protein Homolog 1, TWSG1, TSG

Molecular Weight: 23.18 kDa

UniProt: Q9GZX9