HIBCH Protein (His tag)
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- Target See all HIBCH Proteins
- HIBCH (3-hydroxyisobutyryl-CoA Hydrolase (HIBCH))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HIBCH protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product HIBCH Protein
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3-hydroxyisobutyryl-CoA Hydrolase (HIBCH) (AA 33-386) protein (His tag)
HIBCH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
3-hydroxyisobutyryl-CoA Hydrolase (HIBCH) (AA 1-338) protein (GST tag)HIBCH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
3-hydroxyisobutyryl-CoA Hydrolase (HIBCH) (AA 33-386) protein (His tag)Crystallography grade HIBCH Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
3-hydroxyisobutyryl-CoA Hydrolase (HIBCH) (AA 33-385) protein (His tag)Crystallography grade HIBCH Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
3-hydroxyisobutyryl-CoA Hydrolase (HIBCH) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)HIBCH Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial, HIBYLCOAH
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0)containing 10% glycerol 0.2 M NaCl, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- HIBCH (3-hydroxyisobutyryl-CoA Hydrolase (HIBCH))
- Alternative Name
- HIBCH (HIBCH Products)
- Synonyms
- DDBDRAFT_0189353 Protein, DDBDRAFT_0233831 Protein, DDB_0189353 Protein, DDB_0233831 Protein, T11J7.4 Protein, T11J7_4 Protein, T11J7.5 Protein, T11J7_5 Protein, HIBYLCOAH Protein, 2610509I15Rik Protein, AI648812 Protein, zgc:110824 Protein, 3-hydroxyisobutyryl-coenzyme A hydrolase Protein, 3-hydroxyisobutyryl-coenzyme a hydrolase,putative Protein, 3-hydroxyisobutyryl-Coenzyme A hydrolase Protein, 3-hydroxyisobutyryl-CoA hydrolase (predicted) Protein, 3-hydroxyisobutyryl-CoA hydrolase Protein, ATP-dependent caseinolytic (Clp) protease/crotonase family protein Protein, putative 3-hydroxyisobutyryl-coenzyme A hydrolase Protein, 3-hydroxyisobutyryl-CoA hydrolase L homeolog Protein, PVX_100950 Protein, CpipJ_CPIJ012030 Protein, PKH_145800 Protein, hibch Protein, MCYG_05070 Protein, SPBC2D10.09 Protein, CNA02940 Protein, LACBIDRAFT_292320 Protein, SJAG_00944 Protein, VDBG_07520 Protein, AT2G30650 Protein, AT2G30660 Protein, LBRM_32_3920 Protein, HIBCH Protein, Hibch Protein, hibch.L Protein
- Background
- HIBCH is the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants. Recombinant human HIBCH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 42.1kDa(379aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_055177
- Pathways
- Monocarboxylic Acid Catabolic Process
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