TCEA1 Protein (His tag)
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- Target See all TCEA1 Proteins
- TCEA1 (Transcription Elongation Factor A Protein 1 (TCEA1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TCEA1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product TCEA1 Protein
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Transcription Elongation Factor A Protein 1 (TCEA1) (AA 1-301) protein (His tag)
TCEA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Transcription Elongation Factor A Protein 1 (TCEA1) (AA 1-301) protein (GST tag)TCEA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Transcription Elongation Factor A Protein 1 (TCEA1) (AA 81-185) protein (GST tag)TCEA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Transcription Elongation Factor A Protein 1 (TCEA1) (AA 1-301) protein (Strep Tag)Crystallography grade TCEA1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Transcription Elongation Factor A Protein 1 (TCEA1) (AA 1-301) protein (Strep Tag)Crystallography grade TCEA1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Comment
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Synonyms: Transcription elongation factor A protein 1, GTF2S, SII, TCEA, TF2S, TFIIS
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer, pH7.5, 20% glycerol, 1 MM DTT, 50 mM NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- TCEA1 (Transcription Elongation Factor A Protein 1 (TCEA1))
- Alternative Name
- TCEA1 (TCEA1 Products)
- Synonyms
- gtf2s Protein, sii Protein, tcea Protein, tf2s Protein, TCEA1 Protein, S-II Protein, GTF2S Protein, SII Protein, TCEA Protein, TF2S Protein, TFIIS Protein, fj48e12 Protein, wu:fj48e12 Protein, zgc:63975 Protein, transcription elongation factor A (SII), 1 Protein, transcription elongation factor A1 Protein, transcription elongation factor A (SII) 1 Protein, transcription elongation factor A (SII), 1 L homeolog Protein, tcea1 Protein, TCEA1 Protein, Tcea1 Protein, tcea1.L Protein
- Background
- TCEA1 (Transcription elongation factor A protein 1) belongs to the TFS-II family. TCEA1 binds to Pol II and functions to cleave the nascent transcript, thereby unlocking the complex and allowing transcription to continue. Localized to the nucleus, TCEA1 contains three independently-folding domains, all of which are necessary for proper binding to Pol II. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Recombinant human TCEA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.5kDa (325aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_006747
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