SNF8 Protein (His tag)
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- Target See all SNF8 Proteins
- SNF8 (Vacuolar-sorting Protein SNF8 (SNF8))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SNF8 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product SNF8 Protein
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Vacuolar-sorting Protein SNF8 (SNF8) (AA 1-258) protein (His tag)
SNF8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Vacuolar-sorting Protein SNF8 (SNF8) (AA 1-258) protein (GST tag)SNF8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Vacuolar-sorting Protein SNF8 (SNF8) (AA 23-122) protein (GST tag)SNF8 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Vacuolar-sorting Protein SNF8 (SNF8) (AA 1-258) protein (Strep Tag)Crystallography grade SNF8 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Vacuolar-sorting Protein SNF8 (SNF8) protein (Myc-DYKDDDDK Tag)SNF8 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Vacuolar-sorting protein SNF8, Dot3, EAP30, VPS22
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer, pH 8.0, 50% glycerol, 2 mM DTT, 200 mM NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- SNF8 (Vacuolar-sorting Protein SNF8 (SNF8))
- Alternative Name
- SNF8 (SNF8 Products)
- Synonyms
- d11moh34 Protein, vps22 Protein, Dot3 Protein, EAP30 Protein, VPS22 Protein, D11moh34 Protein, RGD1310144 Protein, D11Moh34 Protein, D11MOH34 Protein, zgc:101578 Protein, SNF8, ESCRT-II complex subunit Protein, eap30 subunit of ell complex, putative Protein, EAP30 subunit of ELL complex Protein, SNF8, ESCRT-II complex subunit S homeolog Protein, SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) Protein, snf8 Protein, TA17290 Protein, TVAG_107410 Protein, Bm1_05145 Protein, SNF8 Protein, Snf8 Protein, snf8.S Protein
- Background
- SNF 8, also known as EAP30 (ELL-associated protein of 30 kDa), is localizes to both the nucleus and the cytoplasm and is a member of the SNF8 family of vacuolar sorting proteins. This protein is subunit of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Recombinant human SNF8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 31.4kDa (282aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_009172
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