CMBL Protein (His tag)
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- Target See all CMBL Proteins
- CMBL (Carboxymethylenebutenolidase Homolog (CMBL))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CMBL protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product CMBL Protein
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Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (His tag)
CMBL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (GST tag)CMBL Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Carboxymethylenebutenolidase Homolog (CMBL) (AA 2-245) protein (His tag)Crystallography grade CMBL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Carboxymethylenebutenolidase Homolog (CMBL) (AA 2-245) protein (His tag)Crystallography grade CMBL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Carboxymethylenebutenolidase Homolog (CMBL) protein (Myc-DYKDDDDK Tag)CMBL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Carboxymethylenebutenolidase homolog
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM EDTA
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- CMBL (Carboxymethylenebutenolidase Homolog (CMBL))
- Alternative Name
- CMBL (CMBL Products)
- Synonyms
- JS-1 Protein, RGD1306952 Protein, 2310016A09Rik Protein, carboxymethylenebutenolidase homolog Protein, carboxymethylenebutenolidase-like (Pseudomonas) Protein, CMBL Protein, Cmbl Protein
- Background
- Carboxymethylenebutenolidase homolog, also known as CMBL, is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene that maps to human chromosome 5p15.2. Recombinant human CMBL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 30.6 kDa (269aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_620164
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