MPST Protein (His tag)
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- Target See all MPST Proteins
- MPST (Mercaptopyruvate Sulfurtransferase (MPST))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MPST protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product MPST Protein
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- Comment
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Synonyms: 3-mercaptopyruvate sulfurtransferase, MST, TST2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 20% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- MPST (Mercaptopyruvate Sulfurtransferase (MPST))
- Alternative Name
- MPST (MPST Products)
- Synonyms
- MST Protein, TST2 Protein, Mst Protein, mst Protein, tst Protein, tst2 Protein, fa96h11 Protein, mpst Protein, wu:fa96h11 Protein, mercaptopyruvate sulfurtransferase Protein, zgc:162544 Protein, mercaptopyruvate sulfurtransferase L homeolog Protein, mercaptopyruvate sulfurtransferase S homeolog Protein, MPST Protein, Mpst Protein, mpst Protein, zgc:162544 Protein, sseA Protein, mpst.L Protein, mpst.S Protein
- Background
- MPST catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. Recombinant human MPST protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 35 kDa (321aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_001013454
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