EGLN3 Protein (His tag)
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- Target See all EGLN3 Proteins
- EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EGLN3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by using conventional chromatography.
- Top Product
- Discover our top product EGLN3 Protein
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Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 1-239) protein (His tag)
EGLN3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 1-239) protein (GST tag)EGLN3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 94-193) protein (GST tag)EGLN3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 1-239) protein (Strep Tag)Crystallography grade EGLN3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 1-239) protein (Strep Tag)Crystallography grade EGLN3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 2-239) protein (His tag)EGLN3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) protein (Myc-DYKDDDDK Tag)EGLN3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Egl nine homolog 3, HIFPH3, PHD3
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5 MM DTT, 2 mM EDTA
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))
- Alternative Name
- EGLN3 (EGLN3 Products)
- Synonyms
- zgc:77019 Protein, wu:fj78a08 Protein, EGLN3 Protein, phd3 Protein, PHD-3 Protein, PHD3 Protein, SM-20 Protein, 2610021G09Rik Protein, AI505553 Protein, AI648162 Protein, Hif-p4h-3 Protein, Phd3 Protein, HIFP4H3 Protein, HIFPH3 Protein, egl-9 family hypoxia-inducible factor 3 Protein, egl-9 family hypoxia inducible factor 3 Protein, egl-9 family hypoxia-inducible factor 3 L homeolog Protein, egln3 Protein, EGLN3 Protein, egln3.L Protein, Egln3 Protein
- Background
- EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 29.8 kDa (263aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_071356
- Pathways
- Positive Regulation of Endopeptidase Activity
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