IL-4 Protein

Catalog No. ABIN1305127

Recombinant IL-4 protein expressed in Escherichia coli (E. coli) and has been mentioned in 2+ publications.

Quick Overview for IL-4 Protein (ABIN1305127)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Purity: ≥ 95 %

Product Details

Brand: BD Pharmingen™

Specificity: Biological Activity: Proliferation is measured using MC/9 indicator cells pulsed with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide).
Specific Activity: 0.3 - 2.0 x 10^8 Units/mg
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.
ED50: 50 - 300 pg/mL

Characteristics: Recombinant mouse IL-4 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, pH 7.2, containing glycerol and 2.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Recombinant mouse IL-4 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers Lambert Law.

Purification: Immunoaffinity Chromatography
Recombinant mouse IL-4 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IL-4 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of mouse IL-4, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Mouse IL-4 is useful as a quantitative standard for measuring mouse IL-4 protein levels in an IL-4 specific sandwich ELISA with the purified 11B11 antibody (Cat. No. 554434) as a capture antibody and the biotinylated BVD6-24G2 (Cat. No. 554390) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-4 standard from ~2,000 to 15 pg/mL should be included in each ELISA plate. For assay of mouse IL-4 in complex fluids such as serum or plasma, the BD OptEIA™ Mouse IL-4 ELISA Set is recommended (Cat. No. 555198).

Comment:

BD Pharmingen™ Recombinant Mouse IL-4 - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interleukin-4 (IL-4) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes. IL-4 is responsible for activities previously assigned to BCGF, BCDF, BSF-2 and TCGF-2. Mouse IL-4 is a 14 kD protein containing 120 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Paul: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, (1991) (PubMed).

Noma, Sideras, Naito, Bergstedt-Lindquist, Azuma, Severinson, Tanabe, Kinashi, Matsuda, Yaoita: "Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter." in: Nature, Vol. 319, Issue 6055, pp. 640-6, (1986) (PubMed).

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4

Background: Interleukin-4 (IL-4) is a species-specific cytokine which promotes the proliferation, differentiation and cell-surface protein modulation of B lymphocytes. IL-4 is responsible for activities previously assigned to BCGF, BCDF, BSF-2 and TCGF-2. Mouse IL-4 is a 14 kD protein containing 120 amino acid residues.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation