Fission 1 Protein (FIS1) (AA 1-122) (His tag)

Catalog No. ABIN1692017

Product Details

Target: Fission 1 (FIS1)

Protein Type: Recombinant

Protein Characteristics: AA 1-122

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Fission 1 protein is labelled with His tag.

Purpose: Recombinant Human Mitochondrial Fission 1 Protein/FIS1 (C-6His)

Sequence: MEAVLNELVS VEDLLKFEKK FQSEKAAGSV SKSTQFEYAW CLVRSKYNDD IRKGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT EPQNNQAKEL ERLIDKAMKK DGVEHHHHHH

Characteristics: Recombinant Human Mitochondrial Fission 1 Protein/FIS1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gly122) of Human FIS1 fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: Fission 1 (FIS1)

Alternative Name: FIS1 (FIS1 Products)

Synonyms: TTC11 Protein, 2010003O14Rik Protein, Ttc11 Protein, fission, mitochondrial 1 Protein, FIS1 Protein, Fis1 Protein

Sub Type: Fusionprotein

Background: Mitochondrial Fission 1 Protein (FIS1) is a member of the FIS1 family. FIS1 is a single-pass membrane protein and contains one TPR repeat. FIS1 is part of the mitochondrial complex that promotes mitochondrial fission. FIS1 can induce cytochrome C discharge from the mitochondrion to the cytosol, eventually leading to apoptosis. In addition, FIS1 participates in peroxisomal growth and division. The C-terminus of FIS1 is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.
Alternative Names: Mitochondrial Fission 1 Protein, FIS1 Homolog, hFis1, Tetratricopeptide Repeat Protein 11, TPR Repeat Protein 11, FIS1, TTC11, CGI-135

Molecular Weight: 15.2 kDa

UniProt: Q9Y3D6

Pathways: Positive Regulation of Endopeptidase Activity