RNF146 Protein (GST tag)
(1 reference)-
- Target See all RNF146 Proteins
- RNF146 (Ring Finger Protein 146 (RNF146))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RNF146 protein is labelled with GST tag.
- Application
- Pull-Down Assay (Pull-Down)
- Specificity
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WWE Negative Control Resin, ABIN1741735 is identical to the ABIN1741734 resin except for R163A substitution, which effectively abolishes PAR binding. The negative control resin is useful to control for non-specific binding, and its use is optional.
WWE Neg Control Resin, 0.5 mL (0.5 mg fusion protein) supplied as a slurry containing approx. 50 µL resin. - Characteristics
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WWE Affinity Resin (ABIN1741734) and Negative Control Resin (ABIN1741735) are designed for the isolation and study of poly-ADP-ribosylated (PARylated) proteins. Through the use of this highly specific PAR affinity resin, PARylated proteins are isolated from cell or tissue lysates without the use of anti-PAR antibodies.
RNF146-175 - Purification
- Affinity chromatography
- Purity
- > 95 %
- Top Product
- Discover our top product RNF146 Protein
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Ring Finger Protein 146 (RNF146) (AA 1-358) protein (GST tag)
RNF146 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ring Finger Protein 146 (RNF146) (AA 1-110) protein (GST tag)RNF146 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ring Finger Protein 146 (RNF146) (AA 1-359) protein (Strep Tag)Crystallography grade RNF146 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ring Finger Protein 146 (RNF146) (AA 1-359) protein (Strep Tag)Crystallography grade RNF146 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- 20 µL=20 µg per reaction
- Comment
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70.00
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 10 mM sodium phosphate, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, and 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze!
- Storage
- 4 °C
- Storage Comment
- Stable for 6 months from date of shipment when stored at 4 ˚C.
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: "Nutritional energy stimulates NAD+ production to promote tankyrase-mediated PARsylation in insulinoma cells." in: PLoS ONE, Vol. 10, Issue 4, pp. e0122948, (2015) (PubMed).
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: "Nutritional energy stimulates NAD+ production to promote tankyrase-mediated PARsylation in insulinoma cells." in: PLoS ONE, Vol. 10, Issue 4, pp. e0122948, (2015) (PubMed).
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- Target
- RNF146 (Ring Finger Protein 146 (RNF146))
- Alternative Name
- RNF146 (RNF146 Products)
- Synonyms
- RP3-351K20.1 Protein, dJ351K20.1 Protein, 2610509H23Rik Protein, Iduna Protein, RNF146 Protein, rnf146 Protein, dactylidin Protein, wu:fi29e08 Protein, zgc:55909 Protein, ring finger protein 146 Protein, RING finger protein 146 Protein, ring finger protein 146 S homeolog Protein, RNF146 Protein, Rnf146 Protein, rn146 Protein, rnf146.S Protein, rnf146 Protein
- Background
- RNF146 (Iduna) is a RING-domain E3 ubiquitin ligase that positively regulates Wnt signalling. RNF146 directly interacts with poly(ADP-ribose) through its WWE domain. The WWE domain is a conserved globular domain found in multiple PARPs and E3 ligases.
- Molecular Weight
- 8 kDa + GST
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