KDM5B Protein (full length) (DYKDDDDK Tag)
-
- Target See all KDM5B Proteins
- KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))
- Protein Type
- Recombinant
- Protein Characteristics
- full length
-
Origin
- Human
-
Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This KDM5B protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant JARID1B / KDM5B (accession number NP_006609, full length) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 179.9 kDa.
-
Lysine (K)-Specific Demethylase 5B (KDM5B) (AA 231-319) protein (GST tag)
KDM5B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lysine (K)-Specific Demethylase 5B (KDM5B) (AA 1-1544) protein (Strep Tag)Crystallography grade KDM5B Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Lysine (K)-Specific Demethylase 5B (KDM5B) (AA 1-1544) protein (Strep Tag)Crystallography grade KDM5B Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
-
- Application Notes
- Recombinant JARID1B / KDM5B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Catalytic Rate: 20 turnovers/ enzyme molecule/ hour. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 50 nM Recombinant JARID1B / KDM5B protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Restrictions
- For Research Use only
-
- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
-
- Target
- KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))
- Alternative Name
- JARID1B / KDM5B (KDM5B Products)
- Synonyms
- jarid1b Protein, jarid1ba Protein, id:ibd5050 Protein, wu:fd15c05 Protein, cb264 Protein, jarid1bb Protein, plu1 Protein, wu:fc44h11 Protein, zgc:85741 Protein, JARID1B Protein, CT31 Protein, PLU-1 Protein, PLU1 Protein, PUT1 Protein, RBBP2H1A Protein, 2010009J12Rik Protein, 2210016I17Rik Protein, AW556288 Protein, D1Ertd202e Protein, Jarid1b Protein, Plu1 Protein, Rb-Bp2 Protein, mKIAA4034 Protein, RGD1565602 Protein, lysine (K)-specific demethylase 5Ba Protein, lysine (K)-specific demethylase 5Bb Protein, lysine demethylase 5B Protein, lysine (K)-specific demethylase 5B Protein, kdm5ba Protein, kdm5bb Protein, KDM5B Protein, Kdm5b Protein
- Background
- Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma.
- Molecular Weight
- 179.9 kDa
- Pathways
- Warburg Effect
-
