KDM5C Protein (DYKDDDDK Tag)
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- Target See all KDM5C Proteins
- KDM5C (Lysine (K)-Specific Demethylase 5C (KDM5C))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This KDM5C protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant JARID1C / KDM5C (accession number NP_004178.2) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 180 kDa.
- Top Product
- Discover our top product KDM5C Protein
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Lysine (K)-Specific Demethylase 5C (KDM5C) (AA 1-1560) protein (Strep Tag)
Crystallography grade KDM5C Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Lysine (K)-Specific Demethylase 5C (KDM5C) (AA 1-1554) protein (Strep Tag)Crystallography grade KDM5C Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
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- Application Notes
- Recombinant JARID1C / KDM5C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JARID1C / KDM5C protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- KDM5C (Lysine (K)-Specific Demethylase 5C (KDM5C))
- Alternative Name
- JARID1C / KDM5C (KDM5C Products)
- Synonyms
- DXS1272E Protein, JARID1C Protein, MRXJ Protein, MRXSCJ Protein, MRXSJ Protein, SMCX Protein, XE169 Protein, Jarid1c Protein, Smcx Protein, mKIAA0234 Protein, histone demethylase JARID1C Protein, lysine-specific demethylase 5C Protein, lysine demethylase 5C Protein, lysine (K)-specific demethylase 5C Protein, MGYG_04221 Protein, LOC100075501 Protein, KDM5C Protein, kdm5c Protein, Kdm5c Protein
- Background
- Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. It is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5C participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and CoREST pAb at neuron-restrictive silencer elements. Mutations in KDM5C have been associated with X-linked mental retardation.
- Molecular Weight
- 180 kDa
- Pathways
- Warburg Effect
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