KDM4C Protein (DYKDDDDK Tag)
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- Target See all KDM4C Proteins
- KDM4C (Lysine (K)-Specific Demethylase 4C (KDM4C))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Baculovirus
- Purification tag / Conjugate
- This KDM4C protein is labelled with DYKDDDDK Tag.
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant JMJD2C / KDM4C (accession number NP_055876.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a calculated molecular weight of 124.1 kDa.
- Top Product
- Discover our top product KDM4C Protein
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Lysine (K)-Specific Demethylase 4C (KDM4C) (AA 1-1056) protein (His tag)
Crystallography grade KDM4C Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Lysine (K)-Specific Demethylase 4C (KDM4C) (AA 1-1054) protein (His tag)Crystallography grade KDM4C Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Lysine (K)-Specific Demethylase 4C (KDM4C) protein (Myc-DYKDDDDK Tag)KDM4C Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Recombinant JMJD2C / KDM4C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 and H3K36me3 demethylase. Catalytic Activity: >100 turnovers/ enzyme molecule Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 60 nM Recombinant JMJD2C / KDM4C protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- KDM4C (Lysine (K)-Specific Demethylase 4C (KDM4C))
- Alternative Name
- JMJD2C / KDM4C (KDM4C Products)
- Background
- KDM4C (lysine (K)-specific demethylase 4C), also known as JMJD2C (Jumonji Domain Containing 2C) is a nuclear protein that functions as a trimethylation-specific histone demethylase that preferentially demethylates trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated form. KDM4C has no activity for mono- and dimethylated H3K9 and H3K36. Chromosomal aberrations and overexpression of KDM4C are associated with esophageal squamous cell carcinoma. KDM4C regulates colonosphere formation in colorectal cancers by mediating the crosstalk between Wnt and Notch signaling pathways.
- Molecular Weight
- 124.1 kDa
- Pathways
- Nuclear Hormone Receptor Binding, Warburg Effect
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