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CD3 Protein (CD3) (His tag)

CD3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN2713619
  • Target See all CD3 Proteins
    CD3
    Protein Type
    Recombinant
    Origin
    Human
    Source
    • 2
    HEK-293 Cells
    Purification tag / Conjugate
    This CD3 protein is labelled with His tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human CD3 protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 95 % as determined by SDS-PAGE and Coomassie blue staining
    Endotoxin Level
    Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
    Top Product
    Discover our top product CD3 Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Buffer
    Lyophilized from a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2
    Handling Advice
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    CD3
    Alternative Name
    CD3 (CD3 Products)
    Synonyms
    CD3 Protein, AI504783 Protein, CD3epsilon Protein, T3e Protein, cd3-gamma Protein, cd3g Protein, cd3g-a Protein, cd3g/d Protein, cd3gd Protein, t3g Protein, CD3e molecule Protein, CD3d molecule Protein, CD3 antigen, epsilon polypeptide Protein, CD3g molecule S homeolog Protein, CD3E Protein, CD3D Protein, Cd3e Protein, cd3g.S Protein
    Background
    The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
    Molecular Weight
    12.79kD
    NCBI Accession
    NP_000724
    Pathways
    TCR Signaling, Ubiquitin Proteasome Pathway
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