CRYBA1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715237

Product Details

Target: CRYBA1 (Crystallin, beta A1 (CRYBA1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CRYBA1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Beta-crystallin A3 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CRYBA1 (Crystallin, beta A1 (CRYBA1))

Alternative Name: beta-Crystallin a3 (CRYBA1 Products)

Synonyms: CRYB1 Protein, CTRCT10 Protein, BA3/A1 Protein, Cryb Protein, BA3A1C Protein, beta-A3 Protein, cryba1 Protein, zgc:92688 Protein, CRYBA3 Protein, cryb1 Protein, zgc:92720 Protein, crystallin beta A1 Protein, crystallin, beta A1 Protein, crystallin, beta A1a Protein, crystallin beta A1 L homeolog Protein, crystallin, beta A1b Protein, CRYBA1 Protein, Cryba1 Protein, cryba1a Protein, cryba1.L Protein, cryba1 Protein, cryba1b Protein

Background: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, encodes two proteins (crystallin, beta A3 and crystallin, beta A1) from a single mRNA, the latter protein is 17 aa shorter than crystallin, beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'.

Molecular Weight: 25 kDa

NCBI Accession: NP_005199