NDUFA12 Protein (Myc-DYKDDDDK Tag)
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- Target See all NDUFA12 Proteins
- NDUFA12 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 12 (NDUFA12))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NDUFA12 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human DAP13 / NDUFA12 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NDUFA12 Protein
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NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 12 (NDUFA12) (AA 1-145) protein (GST tag)
NDUFA12 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 12 (NDUFA12) (AA 1-145) protein (Strep Tag)Crystallography grade NDUFA12 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 12 (NDUFA12) (AA 1-145) protein (Strep Tag)Crystallography grade NDUFA12 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- NDUFA12 (NADH Dehydrogenase (Ubiquinone) 1 alpha Subcomplex, 12 (NDUFA12))
- Alternative Name
- Dap13,ndufa12 (NDUFA12 Products)
- Synonyms
- RGD1311462 Protein, si:dkey-183c16.3 Protein, zgc:112053 Protein, B17.2 Protein, DAP13 Protein, 2410011G03Rik Protein, AW112974 Protein, NADH:ubiquinone oxidoreductase subunit A12 Protein, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 Protein, Ndufa12 Protein, NDUFA12 Protein, ndufa12 Protein
- Background
- This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 16.9 kDa
- NCBI Accession
- NP_061326
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