EDDM3B Protein (Myc-DYKDDDDK Tag)
EDDM3B
Origin: Human
Host: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
1 image
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- Target See all EDDM3B products
- EDDM3B (Epididymal Protein 3B (EDDM3B))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This EDDM3B protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Family with sequence similarity 12, member B (epididymal) (FAM12B) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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Epididymal Protein 3B (EDDM3B) (AA 26-147) protein (His tag)
Crystallography grade EDDM3B Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- EDDM3B (Epididymal Protein 3B (EDDM3B))
- Alternative Name
- Family with Sequence Similarity 12, Member B (Epididymal) (Fam12b) (EDDM3B Products)
- Synonyms
- EP3B Protein, HE3B Protein, FAM12B Protein, HE3-BETA Protein, epididymal protein 3B Protein, EDDM3B Protein
- Background
- Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells.
- Molecular Weight
- 17.4 kDa
- NCBI Accession
- NP_071755
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