GRB2-Related Adaptor Protein (GRAP) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2722222, Supplier: Log in to see
Protein Name
  • 8430435N19Rik
  • BB233594
  • GRAP
  • grap
  • zgc:110202
  • MGC109892
  • si:dkeyp-9d4.1
  • Grap
  • GRB2-related adaptor protein
  • GRB2-related adapter protein
  • GRB2-related adaptor protein a
  • GRB2-related adaptor protein b
  • GRAP
  • Grap
  • LOC489534
  • grapa
  • grapb
  • LOC100727005
Origin
Human
2
1
1
Source
HEK-293 Cells
2
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This GRAP protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
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Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human GRAP protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Background This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.
Molecular Weight 25.2 kDa
NCBI Accession NP_006604
Research Area Signaling
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Western Blotting (WB) image for GRB2-Related Adaptor Protein (GRAP) protein (Myc-DYKDDDDK Tag) (ABIN2722222) Validation with Western Blot
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