GTF2A2 Protein (His tag)

Catalog No. ABIN2722303

Product Details

Target: GTF2A2 (General Transcription Factor IIA, 2, 12kDa (GTF2A2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GTF2A2 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human GTF2A2 / TF2A2 (full length, N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: GTF2A2 (General Transcription Factor IIA, 2, 12kDa (GTF2A2))

Alternative Name: Gtf2a2,tf2a2 (GTF2A2 Products)

Synonyms: CG11639 Protein, Dmel\\CG11639 Protein, EG:BACR7A4.7 Protein, TFIIA Protein, tfiia-s-2 Protein, GTF2A2 Protein, DDBDRAFT_0186451 Protein, DDBDRAFT_0216276 Protein, DDB_0186451 Protein, DDB_0216276 Protein, zgc:110584 Protein, TFIIA-gamma Protein, TfIIg Protein, Tfiia2 Protein, HsT18745 Protein, TF2A2 Protein, LOC100136048 Protein, T2AG Protein, gtf2a2 Protein, CG11639 gene product from transcript CG11639-RA Protein, general transcription factor IIA subunit 2 Protein, transcription factor IIA, small chain Protein, general transcription factor IIA, 2 Protein, general transcription factor II A, 2 Protein, transcription initiation factor IIA subunit 2 Protein, TfIIA-S-2 Protein, GTF2A2 Protein, gtf2a2 Protein, Gtf2a2 Protein

Background: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM, Mar 2008].

Molecular Weight: 12.3 kDa

NCBI Accession: NP_004483