HECT Domain Containing 3 (HECTD3) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2722580, Supplier: Log in to see
Protein Name
  • 1700064K09Rik
  • AI467540
  • AW743312
  • RP11-69J16.1
  • HECT domain containing 3
  • HECT domain containing E3 ubiquitin protein ligase 3
  • LOC100222219
  • LOC100348154
  • Hectd3
  • HECTD3
Origin
Human
2
1
Source
HEK-293 Cells
2
1
Protein Type
Recombinant
Purification tag / Conjugate
Myc-DYKDDDDK Tag
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
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Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human HECTD3 protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Background The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8.
Molecular Weight 96.9 kDa
NCBI Accession NP_078878
Research Area Proteolysis / Ubiquitin
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Western Blotting (WB) image for HECT Domain Containing 3 (HECTD3) protein (Myc-DYKDDDDK Tag) (ABIN2722580) Validation with Western Blot
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