HMGCL Protein (Myc-DYKDDDDK Tag)
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- Target See all HMGCL Proteins
- HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HMGCL protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human HMG-CoA lyase / HMGCL protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product HMGCL Protein
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3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 1-325) protein (GST tag)
HMGCL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 28-325) protein (His tag)HMGCL Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 28-325) protein (His tag)Crystallography grade HMGCL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL) (AA 28-325) protein (His tag)Crystallography grade HMGCL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))
- Alternative Name
- Hmg-Coa Lyase,hmgcl (HMGCL Products)
- Synonyms
- Afu7g01720 Protein, AO090038000541 Protein, AW476067 Protein, HL Protein, zgc:56248 Protein, 3-hydroxymethyl-3-methylglutaryl-CoA lyase S homeolog Protein, 3-hydroxymethyl-3-methylglutaryl-CoA lyase Protein, 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase Protein, 3-hydroxy-3-methylglutaryl-Coenzyme A lyase Protein, hmgcl.S Protein, HMGCL Protein, AFUA_7G01720 Protein, NFIA_114450 Protein, ACLA_065820 Protein, AOR_1_910074 Protein, PMAA_004600 Protein, TSTA_103060 Protein, ARB_04874 Protein, TRV_06573 Protein, Hmgcl Protein, hmgcl Protein
- Background
- The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 31.5 kDa
- NCBI Accession
- NP_000182
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