Rubicon Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all Rubicon (KIAA0226) Proteins
- Rubicon (KIAA0226) (KIAA0226)
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Rubicon protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human KIAA0226 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product KIAA0226 Protein
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KIAA0226 (KIAA0226) (AA 10-278) protein (His-IF2DI Tag)
KIAA0226 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses ELISA, WB
KIAA0226 (KIAA0226) (AA 1-375) protein (GST tag)KIAA0226 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
KIAA0226 (KIAA0226) (AA 1-972) protein (Strep Tag)Crystallography grade KIAA0226 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
KIAA0226 (KIAA0226) (AA 1-956) protein (Strep Tag)Crystallography grade KIAA0226 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Rubicon (KIAA0226) (KIAA0226)
- Alternative Name
- Kiaa0226 (KIAA0226 Products)
- Synonyms
- RUBICON Protein, 5330403K09 Protein, Kiaa0226 Protein, Rubicon Protein, mKIAA0226 Protein, RUN and cysteine rich domain containing beclin 1 interacting protein Protein, KIAA0226 Protein, RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein Protein, RUBCN Protein, KIAA0226 Protein, Rubcn Protein
- Background
- The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
- Molecular Weight
- 108.4 kDa
- NCBI Accession
- NP_055502
- Pathways
- Autophagy
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