RPS14 Protein (His tag)
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- Target See all RPS14 Proteins
- RPS14 (Ribosomal Protein S14 (RPS14))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RPS14 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MRPS14 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product RPS14 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- RPS14 (Ribosomal Protein S14 (RPS14))
- Alternative Name
- Mrps14 (RPS14 Products)
- Synonyms
- EMTB Protein, S14 Protein, 2600014J02Rik Protein, AL023078 Protein, fa92e08 Protein, wu:fa92e08 Protein, zgc:73215 Protein, CG1527 Protein, Dmel\CG1527 Protein, RPS14B Protein, RpS14 Protein, RpS14B Protein, S14b Protein, anon-EST:Posey115 Protein, anon-EST:Posey131 Protein, anon-EST:Posey154 Protein, anon-EST:Posey77 Protein, rpS14B Protein, ribosomal protein S14 Protein, 40S ribosomal protein S14 Protein, ribosomal protein S14 L homeolog Protein, 30S ribosomal protein S14 Protein, Ribosomal protein S14b Protein, mitochondrial ribosomal protein S14 Protein, RPS14 Protein, Rps14 Protein, rps-14 Protein, rps14 Protein, rps14.L Protein, LOC100286294 Protein, RpS14 Protein, RpS14b Protein, MRPS14 Protein
- Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants.
- Molecular Weight
- 15.1 kDa
- NCBI Accession
- NP_071383
- Pathways
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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