PICALM Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2728884

Product Details

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 1

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PICALM protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human PICALM (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Zhao, Sagare, Ma, Halliday, Kong, Kisler, Winkler, Ramanathan, Kanekiyo, Bu, Owens, Rege, Si, Ahuja, Zhu, Miller, Schneider, Maeda, Maeda, Sugawara, Ichida, Zlokovic: "Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance." in: Nature neuroscience, Vol. 18, Issue 7, pp. 978-87, (2015) (PubMed).

Target Details

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Alternative Name: Picalm (PICALM Products)

Synonyms: LAP-3 Protein, PEPS Protein, CALM Protein, CLTH Protein, LAP Protein, PAP180 Protein, fit-1 Protein, fit1 Protein, mKIAA4114 Protein, Calm Protein, leucine aminopeptidase 3 Protein, phosphatidylinositol binding clathrin assembly protein Protein, si:ch211-200p22.4 Protein, LAP3 Protein, PICALM Protein, Picalm Protein, si:ch211-200p22.4 Protein

Background: This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(1011)(p13q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 70.6 kDa

NCBI Accession: NP_009097

Pathways: Synaptic Membrane