VAV1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2735197

Product Details

Target: VAV1 (Vav 1 Oncogene (VAV1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This VAV1 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human VAV1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Mahankali, Peng, Henkels, Dinauer, Gomez-Cambronero: "Phospholipase D2 (PLD2) is a guanine nucleotide exchange factor (GEF) for the GTPase Rac2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 49, pp. 19617-22, (2011) (PubMed).

Target Details

Target: VAV1 (Vav 1 Oncogene (VAV1))

Alternative Name: Vav1 (VAV1 Products)

Synonyms: VAV Protein, Vav Protein, vav-T Protein, vav guanine nucleotide exchange factor 1 Protein, vav 1 oncogene Protein, vav guanine nucleotide exchange factor 1 L homeolog Protein, VAV1 Protein, Vav1 Protein, vav1.L Protein

Background: This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Molecular Weight: 98.1 kDa

NCBI Accession: NP_005419

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, BCR Signaling