DNAJB14 Protein (AA 1-379) (Strep Tag)

Catalog No. ABIN3078991

Product Details

Target: DNAJB14 (DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14))

Protein Type: Recombinant

Protein Characteristics: AA 1-379

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DNAJB14 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEGNRDEAEK CVEIAREALN AGNREKAQRF LQKAEKLYPL PSARALLEII MKNGSTAGNS PHCRKPSGSG DQSKPNCTKD STSGSGEGGK GYTKDQVDGV LSINKCKNYY EVLGVTKDAG DEDLKKAYRK LALKFHPDKN HAPGATDAFK KIGNAYAVLS NPEKRKQYDL TGNEEQACNH QNNGRFNFHR GCEADITPED LFNIFFGGGF PSGSVHSFSN GRAGYSQQHQ HRHSGHEREE ERGDGGFSVF IQLMPIIVLI LVSLLSQLMV SNPPYSLYPR SGTGQTIKMQ TENLGVVYYV NKDFKNEYKG MLLQKVEKSV EEDYVTNIRN NCWKERQQKT DMQYAAKVYR DDRLRRKADA LSMDNCKELE RLTSLYKGG
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DNAJB14 (DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14))

Alternative Name: DNAJB14 (DNAJB14 Products)

Synonyms: Dnajb14 Protein, MGC153638 Protein, DKFZp468H1319 Protein, EGNR9427 Protein, PRO34683 Protein, 5730496F10Rik Protein, flj14281 Protein, DnaJ heat shock protein family (Hsp40) member B14 Protein, DnaJ (Hsp40) homolog, subfamily B, member 14 Protein, DnaJ heat shock protein family (Hsp40) member B14 S homeolog Protein, DNAJB14 Protein, dnajb14 Protein, Dnajb14 Protein, dnajb14.S Protein

Background: DnaJ homolog subfamily B member 14,FUNCTION: Acts as a co-chaperone with HSPA8/Hsc70, required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway (PubMed:24732912). Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities (PubMed:24732912). Can also act independently of HSPA8/Hsc70: together with DNAJB12, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers (PubMed:27916661). While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70 (PubMed:27916661). When overexpressed, forms membranous structures together with DNAJB12 and HSPA8/Hsc70 within the nucleus, the role of these structures, named DJANGOs, is still unclear (PubMed:24732912). {ECO:0000269|PubMed:23018488, ECO:0000269|PubMed:24732912, ECO:0000269|PubMed:27916661}., FUNCTION: (Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection (PubMed:21673190, PubMed:24675744). {ECO:0000269|PubMed:21673190, ECO:0000269|PubMed:24675744}.

Molecular Weight: 42.5 kDa

UniProt: Q8TBM8