RPF2 Protein (AA 1-306) (Strep Tag)

Catalog No. ABIN3087443

Product Details

Target: RPF2 (Ribosome Production Factor 2 Homolog (RPF2))

Protein Type: Recombinant

Protein Characteristics: AA 1-306

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RPF2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MDTLDRVVKP KTKRAKRFLE KREPKLNENI KNAMLIKGGN ANATVTKVLK DVYALKKPYG VLYKKKNITR PFEDQTSLEF FSKKSDCSLF MFGSHNKKRP NNLVIGRMYD YHVLDMIELG IENFVSLKDI KNSKCPEGTK PMLIFAGDDF DVTEDYRRLK SLLIDFFRGP TVSNIRLAGL EYVLHFTALN GKIYFRSYKL LLKKSGCRTP RIELEEMGPS LDLVLRRTHL ASDDLYKLSM KMPKALKPKK KKNISHDTFG TTYGRIHMQK QDLSKLQTRK MKGLKKRPAE RITEDHEKKS KRIKKN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RPF2 (Ribosome Production Factor 2 Homolog (RPF2))

Alternative Name: RPF2 (RPF2 Products)

Synonyms: BXDC1 Protein, bA397G5.4 Protein, 2810470K21Rik Protein, AU040229 Protein, AU043242 Protein, Bxdc1 Protein, RPF2 Protein, DDBDRAFT_0184273 Protein, DDBDRAFT_0238377 Protein, DDB_0184273 Protein, DDB_0238377 Protein, F14G24.20 Protein, F14G24_20 Protein, bxdc1 Protein, ik:tdsubc_1a3 Protein, wu:fe17d08 Protein, wu:fj16d06 Protein, xx:tdsubc_1a3 Protein, zgc:77150 Protein, rpf2 Protein, ribosome production factor 2 homolog Protein, brix domain-containing protein Protein, Ribosomal RNA processing Brix domain protein Protein, Brix domain-containing protein Protein, ribosome production factor 2 homolog S homeolog Protein, RPF2 Protein, Rpf2 Protein, bxdc1 Protein, AT3G15460 Protein, AT3G23620 Protein, AT1G52930 Protein, TAGG_RS06720 Protein, LOC9319507 Protein, VDIS_RS04685 Protein, DESMU_RS06690 Protein, MZHIL_RS05915 Protein, rpf2 Protein, rpf2.S Protein

Background: Ribosome production factor 2 homolog (Brix domain-containing protein 1) (Ribosome biogenesis protein RPF2 homolog),FUNCTION: Involved in ribosomal large subunit assembly. May regulate the localization of the 5S RNP/5S ribonucleoprotein particle to the nucleolus. {ECO:0000269|PubMed:24120868}.

Molecular Weight: 35.6 kDa

UniProt: Q9H7B2

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly