ALDH9A1 Protein (AA 1-494) (Strep Tag)

Catalog No. ABIN3088535

Product Details

Target: ALDH9A1 (Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1))

Protein Type: Recombinant

Protein Characteristics: AA 1-494

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ALDH9A1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MSTGTFVVSQ PLNYRGGARV EPADASGTEK AFEPATGRVI ATFTCSGEKE VNLAVQNAKA AFKIWSQKSG MERCRILLEA ARIIREREDE IATMECINNG KSIFEARLDI DISWQCLEYY AGLAASMAGE HIQLPGGSFG YTRREPLGVC VGIGAWNYPF QIASWKSAPA LACGNAMVFK PSPFTPVSAL LLAEIYSEAG VPPGLFNVVQ GGAATGQFLC QHPDVAKVSF TGSVPTGMKI MEMSAKGIKP VTLELGGKSP LIIFSDCDMN NAVKGALMAN FLTQGQVCCN GTRVFVQKEI LDKFTEEVVK QTQRIKIGDP LLEDTRMGPL INRPHLERVL GFVKVAKEQG AKVLCGGDIY VPEDPKLKDG YYMRPCVLTN CRDDMTCVKE EIFGPVMSIL SFDTEAEVLE RANDTTFGLA AGVFTRDIQR AHRVVAELQA GTCFINNYNV SPVELPFGGY KKSGFGRENG RVTIEYYSQL KTVCVEMGDV ESAF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ALDH9A1 (Aldehyde Dehydrogenase 9 Family, Member A1 (ALDH9A1))

Alternative Name: ALDH9A1 (ALDH9A1 Products)

Synonyms: ALDH4 Protein, ALDH7 Protein, ALDH9 Protein, E3 Protein, TMABADH Protein, Ahd-2 Protein, Ahd2 Protein, Aldh1 Protein, Aldh1a2 Protein, E1 Protein, Raldh1 Protein, Tmaba-dh Protein, MGC52697 Protein, aldh9a1 Protein, ALDH9A1 Protein, ECK1438 Protein, JW1439 Protein, prr Protein, ydcW Protein, SC6A9.01c Protein, SC8B7.12c Protein, AA139417 Protein, Abaldh Protein, ESTM40 Protein, TMABA-DH Protein, Tmabadh Protein, aldh9 Protein, aldehyde dehydrogenase 9 family member A1 Protein, aldehyde dehydrogenase family 1, subfamily A1 Protein, aldehyde dehydrogenase 9 family, member A1 Protein, aldehyde dehydrogenase 9 family member A1 S homeolog Protein, aldehyde dehydrogenase 9 family, member A1b Protein, gamma-aminobutyraldehyde dehydrogenase Protein, aldehyde dehydrogenase Protein, aldehyde dehydrogenase 9, subfamily A1 Protein, betaine aldehyde dehydrogenase Protein, ALDH9A1 Protein, Aldh1a1 Protein, Aldh9a1 Protein, aldh9a1.S Protein, aldh9a1b Protein, aldh9a1 Protein, ECs2048 Protein, patD Protein, Z2275 Protein, SCO5666 Protein, SAV_2592 Protein, SMc01656 Protein, STY1467 Protein, ECA1542 Protein, CYA_0364 Protein, CYB_0715 Protein, SGR_RS09015 Protein, ydcW Protein, APA01_RS11285 Protein, CTU_20580 Protein, Sros_1880 Protein

Background: 4-trimethylaminobutyraldehyde dehydrogenase (TMABA-DH) (TMABALDH) (EC 1.2.1.47) (Aldehyde dehydrogenase E3 isozyme) (Aldehyde dehydrogenase family 9 member A1) (EC 1.2.1.3) (Formaldehyde dehydrogenase) (EC 1.2.1.46) (Gamma-aminobutyraldehyde dehydrogenase) (EC 1.2.1.19) (R-aminobutyraldehyde dehydrogenase) [Cleaved into: 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed],FUNCTION: Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency (in vitro). Can catalyze the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction, but with low efficiency. Catalyzes the oxidation of aldehydes arising from biogenic amines and polyamines. {ECO:0000269|PubMed:10702312, ECO:0000269|PubMed:1799975, ECO:0000269|PubMed:30914451, ECO:0000269|PubMed:8645224}.

Molecular Weight: 53.8 kDa

UniProt: P49189