ACSL1 Protein (AA 46-698) (His tag)

Catalog No. ABIN3088624

Product Details

Target: ACSL1 (Acsl1) (Acyl-CoA Synthetase Long-Chain Family Member 1 (Acsl1))

Protein Type: Recombinant

Protein Characteristics: AA 46-698

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This ACSL1 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: TRPKPLKPPC DLSMQSVEVA GSGGARRSAL LDSDEPLVYF YDDVTTLYEG FQRGIQVSNN GPCLGSRKPD QPYEWLSYKQ VAELSECIGS ALIQKGFKTA PDQFIGIFAQ NRPEWVIIEQ GCFAYSMVIV PLYDTLGNEA ITYIVNKAEL SLVFVDKPEK AKLLLEGVEN KLIPGLKIIV VMDAYGSELV ERGQRCGVEV TSMKAMEDLG RANRRKPKPP APEDLAVICF TSGTTGNPKG AMVTHRNIVS DCSAFVKATE NTVNPCPDDT LISFLPLAHM FERVVECVML CHGAKIGFFQ GDIRLLMDDL KVLQPTVFPV VPRLLNRMFD RIFGQANTTL KRWLLDFASK RKEAELRSGI IRNNSLWDRL IFHKVQSSLG GRVRLMVTGA APVSATVLTF LRAALGCQFY EGYGQTECTA GCCLTMPGDW TAGHVGAPMP CNLIKLVDVE EMNYMAAEGE GEVCVKGPNV FQGYLKDPAK TAEALDKDGW LHTGDIGKWL PNGTLKIIDR KKHIFKLAQG EYIAPEKIEN IYMRSEPVAQ VFVHGESLQA FLIAIVVPDV ETLCSWAQKR GFEGSFEELC RNKDVKKAIL EDMVRLGKDS GLKPFEQVKG ITLHPELFSI DNGLLTPTMK AKRPELRNYF RSQIDDLYST IKV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ACSL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ACSL1 (Acsl1) (Acyl-CoA Synthetase Long-Chain Family Member 1 (Acsl1))

Alternative Name: ACSL1 (Acsl1 Products)

Synonyms: ACS1 Protein, FACL1 Protein, FACL2 Protein, LACS Protein, LACS1 Protein, LACS2 Protein, Acas Protein, Acas1 Protein, Acs Protein, FACS Protein, Facl2 Protein, ACS Protein, COAA Protein, zgc:110081 Protein, CER8 Protein, ECERIFERUM 8 Protein, LONG-CHAIN ACYL-COA SYNTHASE 1 Protein, T8I13.8 Protein, F13F21.14 Protein, F13F21_14 Protein, LATERAL ROOT DEVELOPMENT 2 Protein, LRD2 Protein, long-chain acyl-CoA synthetase 2 Protein, acyl-CoA synthetase long chain family member 1 Protein, acyl-CoA synthetase long-chain family member 1 Protein, acyl-CoA synthetase long chain family member 1a Protein, AMP-dependent synthetase and ligase family protein Protein, long-chain acyl-CoA synthetase 2 Protein, ACSL1 Protein, Acsl1 Protein, acsl1a Protein, LACS1 Protein, LACS2 Protein

Background: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.

Molecular Weight: 73.5 kDa Including tag.

UniProt: P33121

Pathways: Regulation of Lipid Metabolism by PPARalpha