Annexin A3 Protein (ANXA3) (AA 2-323) (His tag)
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- Target See all Annexin A3 (ANXA3) Proteins
- Annexin A3 (ANXA3)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-323
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Annexin A3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Sequence
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ASIWVGHRGT VRDYPDFSPS VDAEAIQKAI RGIGTDEKML ISILTERSNA QRQLIVKEYQ AAYGKELKDD LKGDLSGHFE HLMVALVTPP AVFDAKQLKK SMKGAGTNED ALIEILTTRT SRQMKDISQA YYTVYKKSLG DDISSETSGD FRKALLTLAD GRRDESLKVD EHLAKQDAQI LYKAGENRWG TDEDKFTEIL CLRSFPQLKL TFDEYRNISQ KDIVDSIKGE LSGHFEDLLL AIVNCVRNTP AFLAERLHRA LKGIGTDEFT LNRIMVSRSE IDLLDIRTEF KKHYGYSLYS AIKSDTSGDY EITLLKICGG DD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ANXA3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
- Top Product
- Discover our top product ANXA3 Protein
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Annexin A3 (ANXA3) (AA 1-323) protein (GST tag)
ANXA3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Annexin A3 (ANXA3) (AA 2-323) protein (His tag)Crystallography grade ANXA3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Annexin A3 (ANXA3) (AA 5-322), (partial) protein (His tag)ANXA3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Annexin A3 (ANXA3)
- Alternative Name
- ANXA3 (ANXA3 Products)
- Synonyms
- MGC80326 Protein, anxa3 Protein, zgc:101718 Protein, ANXA3 Protein, ANX3 Protein, Anx3 Protein, LC3 Protein, LRRGT00047 Protein, annexin A3 L homeolog Protein, annexin A3a Protein, annexin A3 Protein, Annexin A3 Protein, anxa3.L Protein, anxa3a Protein, ANXA3 Protein, anxa3 Protein, Anxa3 Protein
- Background
- Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.
- Molecular Weight
- 37.2 kDa Including tag.
- UniProt
- P12429
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