ERCC6L2 Protein (AA 1-1561) (His tag)

Catalog No. ABIN3092475

Product Details

Target: ERCC6L2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like 2 (ERCC6L2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1561

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This ERCC6L2 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: MQPGSAPPPG RMDPSAPQPR AETSGKDIWH PGERCLAPSP DNGKLCEASI KSITVDENGK SFAVVLYADF QERKIPLKQL QEVKFVKDCP RNLIFDDEDL EKPYFPNRKF PSSSVAFKLS DNGDSIPYTI NRYLRDYQRE GTRFLYGHYI HGGGCILGDD MGLGKTVQVI SFLAAVLHKK GTREDIENNM PEFLLRSMKK EPLSSTAKKM FLIVAPLSVL YNWKDELDTW GYFRVTVLHG NRKDNELIRV KQRKCEIALT TYETLRLCLD ELNSLEWSAV IVDEAHRIKN PKARVTEVMK ALKCNVRIGL TGTILQNNMK ELWCVMDWAV PGLLGSGTYF KKQFSDPVEH GQRHTATKRE LATGRKAMQR LAKKMSGWFL RRTKTLIKDQ LPKKEDRMVY CSLTDFQKAV YQTVLETEDV TLILQSSEPC TCRSGQKRRN CCYKTNSHGE TVKTLYLSYL TVLQKVANHV ALLQAASTSK QQETLIKRIC DQVFSRFPDF VQKSKDAAFE TLSDPKYSGK MKVLQQLLNH CRKNRDKVLL FSFSTKLLDV LQQYCMASGL DYRRLDGSTK SEERLKIVKE FNSTQDVNIC LVSTMAGGLG LNFVGANVVV LFDPTWNPAN DLQAIDRAYR IGQCRDVKVL RLISLGTVEE IMYLRQIYKQ QLHCVVVGSE NAKRYFEAVQ GSKEHQGELF GIHNLFKFRS QGSCLTKDIL EREGQVEAGI MTATTWLKEG PPAHKLEMPR QPDCQECRGT EQAAEPLAKE ACDLCSDFSD EEPVGATGIK TAKNKAPDSS KASSSPGQLT LLQCGFSKLL ETKCKAVEDS DGNTASDDES SDEQPTCLST EAKDAGCEKN QDSLGTSKHQ KLDNILNPKE KHIFYKSEKI LEQNISSKSD EKKIKNTDKH CILQNVTESE DSDVICPTQY TTERFPDNSI RFKPPLEGSE DSETEHTVKT RNNDNSRNTD DKRNGIISKK LSPENTTLKS ILKRKGTSDI SDESDDIEIS SKSRVRKRAS SLRFKRIKET KKELHNSPKT MNKTNQVYAA NEDHNSQFID DYSSSDESLS VSHFSFSKQS HRPRTIRDRT SFSSKLPSHN KKNSTFIPRK PMKCSNEKVV NQEQSYESMD KFLDGVQEVA YIHSNQNVIG SSKAENHMSR WAAHDVFELK QFSQLPANIA VCSSKTYKEK VDADTLPHTK KGQQPSEGSI SLPLYISNPV NQKKKKVYHT NQTTFIIGET PKGIRRKQFE EMASYFNSSS VNEFAKHITN ATSEERQKML RDFYASQYPE VKEFFVDSVS QFNNSSFEKG EQRTRKKSDK RESLIKPRLS DSETLSFKDS TNKISQVCSL KTYKRKSVKF QNHISYREEV FFNDAETKKS PVSSTQEIDS GKNSQASEDT VTSRSLNSES ETRERRLENT MKDQQDLTRT GISRKEPLLK LENKKIENPV LENTSVISLL GDTSILDDLF KSHGNSPTQL PKKVLSGPME KAKQRPKDFW DILNEQNDES LSKLTDLAVI ETLCEKAPLA APFKRREEPA TSLWKSNEKF LWKKFSPSDT DENATNTQST T
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ERCC6L2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ERCC6L2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like 2 (ERCC6L2))

Alternative Name: ERCC6L2 (ERCC6L2 Products)

Synonyms: 0610007P08Rik Protein, 1700019D06Rik Protein, 9330134C04Rik Protein, Rad26l Protein, Sr278 Protein, C9orf102 Protein, RAD26L Protein, SR278 Protein, C8H9orf102 Protein, RGD1561537 Protein, si:ch211-248d20.2 Protein, BMFS2 Protein, CZH9ORF102 Protein, excision repair cross-complementing rodent repair deficiency, complementation group 6 like 2 Protein, ERCC excision repair 6 like 2 Protein, excision repair cross-complementation group 6-like 2 Protein, excision repair cross-complementation group 6 like 2 Protein, Ercc6l2 Protein, ERCC6L2 Protein, ercc6l2 Protein

Background: May be involved in early DNA damage response. {ECO:0000269|PubMed:24507776}.

Molecular Weight: 178.1 kDa Including tag.

UniProt: Q5T890