FNDC3A Protein (AA 1-1198) (Strep Tag)

Catalog No. ABIN3092646

Product Details

Target: FNDC3A (Fibronectin Type III Domain Containing 3A (FNDC3A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1198

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FNDC3A protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAEHPPLLDT TQILSSDISL LSAPIVSADG TQQVILVQVN PGEAFTIRRE DGQFQCITGP AQVPMMSPNG SVPPIYVPPG YAPQVIEDNG VRRVVVVPQA PEFHPGSHTV LHRSPHPPLP GFIPVPTMMP PPPRHMYSPV TGAGDMTTQY MPQYQSSQVY GDVDAHSTHG RSNFRDERSS KTYERLQKKL KDRQGTQKDK MSSPPSSPQK CPSPINEHNG LIKGQIAGGI NTGSAKIKSG KGKGGTQVDT EIEEKDEETK AFEALLSNIV KPVASDIQAR TVVLTWSPPS SLINGETDES SVPELYGYEV LISSTGKDGK YKSVYVGEET NITLNDLKPA MDYHAKVQAE YNSIKGTPSE AEIFTTLSCE PDIPNPPRIA NRTKNSLTLQ WKAPSDNGSK IQNFVLEWDE GKGNGEFCQC YMGSQKQFKI TKLSPAMGCK FRLSARNDYG TSGFSEEVLY YTSGCAPSMP ASPVLTKAGI TWLSLQWSKP SGTPSDEGIS YILEMEEETS GYGFKPKYDG EDLAYTVKNL RRSTKYKFKV IAYNSEGKSN PSEVVEFTTC PDKPGIPVKP SVKGKIHSHS FKITWDPPKD NGGATINKYV VEMAEGSNGN KWEMIYSGAT REHLCDRLNP GCFYRLRVYC ISDGGQSAVS ESLLVQTPAV PPGPCLPPRL QGRPKAKEIQ LRWGPPLVDG GSPISCYSVE MSPIEKDEPR EVYQGSEVEC TVSSLLPGKT YSFRLRAANK MGFGPFSEKC DITTAPGPPD QCKPPQVTCR SATCAQVNWE VPLSNGTDVT EYRLEWGGVE GSMQICYCGP GLSYEIKGLS PATTYYCRVQ ALSVVGAGPF SEVVACVTPP SVPGIVTCLQ EISDDEIENP HYSPSTCLAI SWEKPCDHGS EILAYSIDFG DKQSLTVGKV TSYIINNLQP DTTYRIRIQA LNSLGAGPFS HMIKLKTKPL PPDPPRLECV AFSHQNLKLK WGEGTPKTLS TDSIQYHLQM EDKNGRFVSL YRGPCHTYKV QRLNESTSYK FCIQACNEAG EGPLSQEYIF TTPKSVPAAL KAPKIEKVND HICEITWECL QPMKGDPVIY SLQVMLGKDS EFKQIYKGPD SSFRYSSLQL NCEYRFRVCA IRQCQDSLGH QDLVGPYSTT VLFISQRTEP PASTNRDTVE STRTRRALSD EQCAAVILVL FAFFSILIAF IIQYFVIK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FNDC3A (Fibronectin Type III Domain Containing 3A (FNDC3A))

Alternative Name: FNDC3A (FNDC3A Products)

Synonyms: FNDC3 Protein, HUGO Protein, bA203I16.1 Protein, bA203I16.5 Protein, 1700094E19Rik Protein, D14Ertd453e Protein, F730017H24Rik Protein, Fndc3 Protein, sys Protein, FNDC3A Protein, DKFZp469E108 Protein, MGC80995 Protein, wu:fk65b04 Protein, fibronectin type III domain containing 3A Protein, fibronectin type III domain containing 3a Protein, fibronectin type III domain containing 3A L homeolog Protein, FNDC3A Protein, Fndc3a Protein, fndc3a.2.L Protein, fndc3a Protein

Background: Fibronectin type-III domain-containing protein 3A (Human gene expressed in odontoblasts),FUNCTION: Mediates spermatid-Sertoli adhesion during spermatogenesis. {ECO:0000250}.

Molecular Weight: 131.9 kDa

UniProt: Q9Y2H6